Robust Nano Cluster Layers for Structural Amplified Fluorescence Biochips

C. Mayer; V. Matyushin; G. Bauer; A. Leitner; F. Aussenegg; T. Schalkhammer

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Robust Nano Cluster Layers for Structural Amplified Fluorescence Biochips

机译：用于结构放大荧光生物芯片的坚固纳米簇层。

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Structural enhanced fluorescence (SEF) amplifies fluorescent signals 200 fold in comparison to standard glass slides. The signal-boosting phenomenon is ideally suited for sensitive biochip applications. DNA biochips based on hybridisation analysis need to cope with chemical and thermal stress, therefore a stable multilayer set up and chemically active surface layers were invented. A palladium layer introduces chemical stability and the necessary structural compatibility for the SEF technique. A silver cluster layer enables the excitation of surface plasmons; sandwich layers of metallic glass establish the proper distance to the fluorophores. The chips are compatible with standard assay procedures of DNA-biochips, and can be reused after hybridisation by stripping in boiling water.

机译：与标准载玻片相比，结构增强的荧光（SEF）可将荧光信号放大200倍。信号增强现象非常适合敏感的生物芯片应用。基于杂交分析的DNA生物芯片需要应对化学和热应力，因此发明了稳定的多层结构和化学活性表面层。钯层引入了SEF技术的化学稳定性和必要的结构兼容性。银团簇层能够激发表面等离激元。金属玻璃的夹层确定了与荧光团的适当距离。该芯片与DNA生物芯片的标准测定程序兼容，杂交后可通过在沸水中汽提来重复使用。

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《Reviews on Advanced Materials Science》 |2003年第1期|共页
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C. Mayer; V. Matyushin; G. Bauer; A. Leitner; F. Aussenegg; T. Schalkhammer;
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1. Dual-color nanoscale assemblies of structurally stable, few-atom silver clusters, as reported by fluorescence resonance energy transfer [J] . Schultz D., Copp S.M., Marke?evi? N., ACS nano . 2013,第11期

机译：结构稳定，原子数少的银团簇的双色纳米级组件，如荧光共振能量转移所报道
2. Dual-color nanoscale assemblies of structurally stable, few-atom silver clusters, as reported by fluorescence resonance energy transfer [J] . Schultz D., Copp S.M., Marke?evi? N., ACS nano . 2013,第11期

机译：结构稳定，原子数少的银团簇的双色纳米级组件，如荧光共振能量转移所报道
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4. Toward Improved Biochips Based on Rolling Circle Amplification—Influences of the Microenvironment on the Fluorescence Properties of Labeled DNA Oligonucleotides [C] . ELKE MAYER-ENTHART, JULIEN SIALELLI, KNUT RURACK, Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging, and Probes . 2008

机译：基于轧制圆形扩增对显微环境对标记DNA寡核苷酸的荧光特性的改进生物芯片
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7. Study of focused-ion-beam–induced structural and compositional modifications in nanoscale bilayer systems by combined grazing incidence x ray reflectivity and fluorescence. [O] . Arac E., Burn D.M., Eastwood D.S., 2012

机译：通过结合掠入射x射线反射率和荧光，研究聚焦离子束在纳米级双层系统中引起的结构和成分修饰。

Robust Nano Cluster Layers for Structural Amplified Fluorescence Biochips

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