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首页> 外文期刊>Revista Brasileira de Fruticultura >Variabilidade e divergência genética entre genótipos de tucumanzeiro-do-pará (Astrocaryum vulgare Mart.) promissores para a produ??o de frutos por marcadores rapd
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Variabilidade e divergência genética entre genótipos de tucumanzeiro-do-pará (Astrocaryum vulgare Mart.) promissores para a produ??o de frutos por marcadores rapd

机译：Tucumanzeiro-do-pará（Astrocaryum vulgare Mart。）基因型之间的遗传变异和差异性有望通过rapd标记产生

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摘要

The objective of this study was to estimate the variability and divergence among fiber palm tree genotypes promising for fruit yield by RAPD markers. Leaf samples from 29 plants, selected in the Active Germplasm Bank from Embrapa Eastern Amazon, were collected based in the fruit yield, but with variations by other characteristics related to production. The samples of these DNA were amplified with 24 primers and analyzed by three multivariate methods using the matrix of genetic dissimilarities obtained by arithmetic complement of the Jaccard index. 332 molecular markers expressing polymorphism of 98.5% were obtained. The RAPD markers presented efficient discriminating power among the 29 evaluated genotypes, determining an average genetic distance among them of 51.7% ranging from 26.9% to 71.5%. The highest mean of distance occurred between genotype 22 and the remaining genotypes, with 66.8%. Eight and fifteen different clusters, possibly heterotic groups were formed by the UPGMA and Tocher methods, respectively. The principal coordinate analyses confirmed the great variability among genotypes. The markers used permitted identification of variability and genetic divergence among genotypes with absence of duplicates, allowing the indication of this material in breeding programs for fruit yield.
机译：这项研究的目的是估计有希望通过RAPD标记获得果实产量的纤维棕榈树基因型之间的变异性和差异性。从29种植物的叶片样品中选出，这些样品是从亚马逊东部Embrapa的活跃种质库中选择的，基于果实的产量，但与生产相关的其他特征也会有所不同。用24个引物扩增这些DNA的样品，并使用Jaccard指数的算术互补获得的遗传差异矩阵通过三种多元方法进行分析。获得了表达多态性为98.5％的332个分子标记。 RAPD标记在29种评估的基因型中表现出有效的区分能力，确定了它们之间的平均遗传距离为51.7％，范围为26.9％至71.5％。基因型22与其余基因型之间的平均距离最高，为66.8％。通过UPGMA和Tocher方法分别形成了八个和十五个不同的簇，可能是杂合基团。主坐标分析证实了基因型之间的巨大差异。所使用的标记可以鉴定基因型之间的变异性和遗传差异，而无需重复，从而可以在育种计划中指明该材料的果实产量。

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