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首页> 外文期刊>Reviews of Reproduction >High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol
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High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

机译：用α-生育酚进行恢复培养后，玻璃化温热的牛成熟卵母细胞具有较高的复活性

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The objective of this study was to investigate whether developmental competence of vitrified–warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l-ascorbic acid or α-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2?h before IVF. The exposure to α-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1–36.3% vs 19.2–25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between α-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in α-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with α-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10?h post-IVF). It was concluded that α-tocopherol treatment of vitrified–warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.
机译：这项研究的目的是研究在恢复培养过程中通过抗氧化剂处理是否可以提高玻璃化加热的牛卵母细胞的发育能力。在实验1中，将两种抗氧化剂之一（1-抗坏血酸或α-生育酚）作为补充添加到恢复培养基中，在IVF之前，将加热后的卵母细胞暴露于该培养基2小时。体外受精后8天，囊胚的产量评估表明，α-生育酚的暴露对拯救卵母细胞有积极作用（35.1–36.3％，未处理的变温后卵母细胞为19.2–25.8％）。就总细胞数和染色体倍性而言，第8天收获的膨胀胚泡的质量在α-生育酚处理的玻璃化组和新鲜对照组之间可比。在实验2中，评估了经α-生育酚处理的变温卵母细胞中活性氧的含量，线粒体活性和皮质颗粒的分布。在这些参数中未发现与对照数据有明显差异。然而，用α-生育酚的处理增加了表现出正常单一紫formation形成的受精卵的百分率（90.3％比未处理的温后卵母细胞的48.0％; IVF后10h）。结论是，α-生育酚处理在恢复培养过程中对玻璃化温热的牛成熟卵母细胞可以提高其复活性，表现为高胚泡产量和较高的平均胚泡总数。

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