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首页> 外文期刊>Reviews of Reproduction >Murine binder of sperm protein homolog 1: a new player in HDL-induced capacitation
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Murine binder of sperm protein homolog 1: a new player in HDL-induced capacitation

机译：精子蛋白同源物的鼠结合剂1：HDL诱导的获能的新参与者

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Binder of sperm (BSP) proteins are ubiquitous among mammals and are exclusively expressed in male genital tract. The main function associated with BSP proteins is their ability to promote sperm capacitation. In mice, two proteins (BSP protein homolog 1 (BSPH1) and BSPH2) have been studied. Using recombinant strategies, BSPH1 was found to bind to epididymal sperm membranes and promote sperm capacitation in vitro. The goal of this study was to evaluate the role of native murine BSPH1 protein in sperm capacitation induced by BSA and HDLs. The effect of antibodies, antigen-binding fragments (Fabs), and F(ab′)2 specific for murine BSPH1 on BSA- and HDL-induced capacitation was tested. Results indicate that BSPH1 has no direct role in BSA-induced capacitation. However, antibodies, Fabs, and F(ab′)2 could block capacitation induced by HDLs and could inhibit the HDL-induced increase in tyrosine phosphorylation, suggesting a specific interaction between HDLs and BSPH1. Results indicate that murine BSPH1 proteins in mice could be a new important piece of the puzzle in sperm capacitation induced by HDLs. As murine BSPH1 is orthologous to human BSPH1, this study could also lead to new insights into the functions and the importance of the human protein in male fertility.
机译：精子结合蛋白（BSP）蛋白在哺乳动物中普遍存在，并且仅在雄性生殖道中表达。与BSP蛋白相关的主要功能是它们促进精子获能的能力。在小鼠中，已经研究了两种蛋白质（BSP蛋白质同源物1（BSPH1）和BSPH2）。使用重组策略，发现BSPH1与附睾的精子膜结合并在体外促进精子获能。这项研究的目的是评估天然小鼠BSPH1蛋白在BSA和HDL诱导的精子获能中的作用。测试了针对鼠BSPH1的抗体，抗原结合片段（Fabs）和F（ab'）2对BSA和HDL诱导的获能的影响。结果表明，BSPH1在BSA诱导的获能中没有直接作用。但是，抗体，Fabs和F（ab'）2可以阻断HDL诱导的获能，并可以抑制HDL诱导的酪氨酸磷酸化增加，提示HDL和BSPH1之间存在特异性相互作用。结果表明，小鼠BSPH1蛋白可能是HDL诱导精子获能的新难题。由于鼠类BSPH1与人类BSPH1是直系同源的，因此这项研究也可能导致对人类蛋白质在男性生育能力中的功能和重要性的新见解。

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