...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista Brasileira de Fruticultura >Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea
【24h】

Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea

机译：西番莲体外发芽基因型对Cow豆蚜传花叶病毒的抗性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SACOMAN, NAYARA NIELE et al. Resistance to Cowpea aphid-borne mosaic virus in in vitro germinated genotypes of Passiflora setacea. Rev. Bras. Frutic. [online]. 2018, vol.40, n.1, e-607. ?Epub?Feb?26, 2018. ISSN 0100-2945.? http://dx.doi.org/10.1590/0100-29452017607. Passion fruit (Passiflora edulis) is a fruit species of great relevance for Brazilian economy. However, it is highly susceptible to the Cowpea aphid-borne mosaic virus (CABMV). The species P.setacea, on the other hand, is resistant to the disease. The present study aimed to identify CABMV-resistant P. setacea genotypes for the introgression of genes into sour passion fruit genetic breeding programs. The seeds of passion fruit genotypes were germinated in vitro in MS culture medium. The seedlings were acclimatized in a growth chamber at the temperature of 27 ± 2oC, photoperiod of 16:8 hours and 80% of relative humidity. Thirty plants of each genotype were mechanically inoculated with extract prepared from leaves collected from passion fruit plants with symptoms of CABMV for the assessment of resistance to CABMV. The severity of leaf symptoms was evaluated by means of a grading scale of visual signs. After the visual evaluation and identification of the asymptomatic genotypes of P. setacea, the PTA-ELISA test was carried out for 30 selected genotypes. According to the visual evaluation, all P. setacea genotypes were highly resistant to CABMV, while the P. edulis genotypes were highly susceptible. Out of the 30 genotypes selected, only PsRJ 4 was considered susceptible by PTA-ELISA. The other genotypes of P. setacea were considered resistant and present great potential for use in passion fruit genetic breeding programs.
机译：SACOMAN，NAYARA NIELE等。菜豆西番莲体外发芽基因型对Cow豆蚜传花叶病毒的抗性。胸罩牧师果然[线上]。 2018年第40卷第1期e-607。 《 Epub》，2018年2月26日。ISSN0100-2945。 http://dx.doi.org/10.1590/0100-29452017607。百香果（Passiflora edulis）是一种与巴西经济息息相关的水果。但是，它对the豆蚜虫传播的花叶病毒（CABMV）非常敏感。另一方面，P.setacea物种对该病具有抗性。本研究旨在鉴定耐CABMV的P. setacea基因型，以便将基因渗入酸性百香果遗传育种程序中。百香果基因型的种子在MS培养基中体外发芽。在温度为27±2oC，光周期为16：8小时和相对湿度为80％的生长室内使幼苗适应环境。机械提取每种基因型的30株植物的提取物，这些提取物是从百香果植物的叶片中提取的，叶片具有CABMV症状，以评估其对CABMV的抗性。叶子症状的严重程度通过视觉症状的分级量表进行评估。在目视评估和鉴定了无定形疟原虫的无症状基因型之后，对30种选择的基因型进行了PTA-ELISA测试。根据视觉评估，所有的P. setacea基因型都对CABMV高度耐药，而P. edulis基因型则高度易感。在选择的30个基因型中，只有PsRJ 4被PTA-ELISA认为是易感的。狗尾草的其他基因型被认为具有抗性，在百香果遗传育种计划中具有巨大的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号