Blood from the umbilical cord is an alternative for the isolation and culture of CD34+ hematopoietic stem cells useful for bone marrow replacement therapy in the treatment o'/> Evaluation of proteins related to the differentiation of CD34+ hematopoietic stem cells from the umbilical cord
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista Cubana de Hematología, Inmunología y Hemoterapia >Evaluation of proteins related to the differentiation of CD34+ hematopoietic stem cells from the umbilical cord
【24h】

Evaluation of proteins related to the differentiation of CD34+ hematopoietic stem cells from the umbilical cord

机译：评估与脐带CD34 +造血干细胞分化有关的蛋白质

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

> face="Verdana" size="2">Blood from the umbilical cord is an alternative for the isolation and culture of CD34+ hematopoietic stem cells useful for bone marrow replacement therapy in the treatment of neoplastic and nonneoplastic pathologies. The expression of proteins related to the differentiation of CD34+ hematopoietic stem cells under culture was evaluated in this paper. The blood samples from the umbilical cord were collected in bags with CPDA (Baxter®) and transported to the Hematology Laboratory of the Faculty of Sciences of the Pontificia Universidad Javeriana. The isolation of the CD34+ hematopoietic stem cells was carried out by using the Miltenyi biotec Microbeads® system starting from mononuclear cells obtained by density gradient with Ficoll-Hypaque Amershan Biosciences®. The CD34+ cells were cultured in Stem Pro® medium supplemented with IL3, SCF and GM-CSF between 5 and 11 days at 37º C and CO2 5 %. Cellular viability was defined. The morphological changes were determined by inverted microscopy, whereas the expression of the CD34, CD33 and CD38 proteins, and of BcI-2 and CD95 was identified by flow citometry. 6 cultures (n=6) enriched with CD34+ hematopoietic cells were established, with a viability average of 84.25 % and a culture time from 5 to 11 days. In the morphological evaluation, it was observed the appearance of cytoplasmatic prolongations or magnupodia-like pseudopodes until the seventh day of culture. The coexpression of the CD34 y CD33 antigens with an increase of CD95 protein from the seventh day on was observed. The CD34+ hematopoietic stem cells taken from the blood of the umbilical cord presented cytoplasmatic correlations that were possibly associated with cell to cell interactions. In our culture system, the obtained hematopoietic stem cells initiated processes of myeloid differentiation that were indirectly related to the expression of BcI-2 and CD95.
机译：> face =“ Verdana” size =“ 2”>脐带血是CD34 +造血干细胞分离和培养的替代方法，可用于骨髓替代疗法，用于治疗肿瘤和非肿瘤性疾病。本文评价了与CD34 +造血干细胞分化相关的蛋白质的表达。来自脐带的血液样本用CPDA（Baxter®）收集在袋子中，并运送到Pontificia Universidad Javeriana科学学院的血液学实验室。通过使用Miltenyi biotecMicrobeads®系统从通过Ficoll-Hypaque AmershanBiosciences®密度梯度获得的单核细胞开始分离CD34 +造血干细胞。将CD34 +细胞在补充了IL3，SCF和GM-CSF的StemPro®培养基中于37ºC和5％CO2下培养5至11天。定义了细胞活力。通过倒置显微镜确定形态变化，而通过流式细胞仪鉴定CD34，CD33和CD38蛋白以及Bcl-2和CD95的表达。建立了6个富含CD34 +造血细胞的培养物（n = 6），平均存活率为84.25％，培养时间为5至11天。在形态学评估中，观察到直到培养的第七天都出现了胞质延长或类似巨噬细胞的伪足。从第七天开始，观察到CD34γCD33抗原与CD95蛋白增加的共表达。从脐带血中提取的CD34 +造血干细胞具有胞质相关性，可能与细胞间的相互作用有关。在我们的培养系统中，获得的造血干细胞启动了髓系分化过程，这与Bcl-2和CD95的表达间接相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号