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首页> 外文期刊>Reviews of Reproduction >Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis
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Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis

机译：标记表达揭示了新生小鼠睾丸中精原细胞的异质性

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Prospermatogonia transition to type A spermatogonia, which provide the source for the spermatogonial stem cell (SSC) pool. A percentage of these type A spermatogonia then differentiate to enter meiosis as spermatocytes by ～P10. It is currently unclear as to when these distinct populations are initially formed in the neonatal testis, and when the expression of markers both characteristic of and required for the adult undifferentiated and differentiating states is established. In this study, we compared expression of known spermatogonial cell fate markers during normal development and in response to the differentiation signal provided by retinoic acid (RA). We found that some markers for the undifferentiated state (ZBTB16/PLZF and CDH1) were expressed in nearly all spermatogonia from P1 through P7. In contrast, differentiation markers (STRA8 and KIT) appeared in a subset of spermatogonia at P4, coincident with the onset of RA signaling. GFRA1, which was present in nearly all prospermatogonia at P1, was only retained in STRA8/KIT? spermatogonia. From P4 through P10, there was a great deal of heterogeneity in the male germ cell population in terms of expression of markers, as markers characteristic of the undifferentiated (except GFRA1) and differentiating states were co-expressed through this interval. After P10, these fate markers diverged to mark distinct populations of undifferentiated and differentiating spermatogonia, and this pattern was maintained in juvenile (P18) and adult (P>60) testes. Taken together, these results reveal that the spermatogonia population is heterogeneous during the first wave of spermatogenesis, and indicate that neonatal spermatogonia may not serve as an ideal substitute for studying the function of adult spermatogonia.
机译：精原细胞转变为A型精原细胞，这为精原干细胞（SSC）库提供了来源。然后，一定比例的这些A型精原细胞以〜P10的形式分化为精母细胞进入减数分裂。目前尚不清楚何时在新生儿睾丸中最初形成这些不同的群体，以及何时确定成年未分化和分化状态的特征性和必需标志物的表达。在这项研究中，我们比较了正常发育过程中视维甲酸（RA）提供的分化信号而对已知精原细胞命运标志物的表达。我们发现一些未分化状态的标记（ZBTB16 / PLZF和CDH1）在从P1到P7的几乎所有精原细胞中都有表达。相反，分化标记物（STRA8和KIT）出现在P4的精原细胞亚群中，与RA信号的发生相吻合。 GFRA1几乎存在于P1的所有生精症中，仅保留在STRA8 / KIT中吗？精原细胞。从P4到P10，在雄性生殖细胞群体中，在标志物的表达上存在很大的异质性，因为未分化的特征性标志物（GFRA1除外）和分化状态在此间隔内共同表达。在P10之后，这些命运标记物分化以标记未分化和分化的精原细胞的不同种群，并且在少年（P18）和成人（P> 60）的睾丸中保持这种模式。综上所述，这些结果表明，在第一代精子发生过程中，精原细胞群是异质的，并表明新生儿精原细胞可能不能作为研究成人精原细胞功能的理想替代品。

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