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首页> 外文期刊>Revista Brasileira de Farmacognosia >Protocol for seaweed decontamination to isolate unialgal cultures
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Protocol for seaweed decontamination to isolate unialgal cultures

机译：海藻去污协议以分离单藻培养物

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Decontamination protocols in seaweeds are essential tools for ecophysiological studies in laboratory cultures. These protocols consist of a set of procedures and physical and chemical treatments that must be adjusted for each species. Thus, the effects of explant size and of combinations of physical treatments (brushing and cutting) and chemical treatments (sodium hypochlorite, detergent, seawater, distilled water, germanium dioxide) on the process of obtaining unialgal culture of two pigmentar morphos of Hypnea musciformis were investigated. It was found that thallus segments 50 mm in length, when transported from the field to the laboratory, remained healthier and were less susceptible to epiphytes than those 7 mm in length. The collected material had surfaces contaminated by diatoms, which were weakly attached, as well as surface contamination caused by strongly attached Sahlingia subintegra. The most efficient combination of physical and chemical treatments was explant brushing, cutting and washing with detergent. This combination eliminated the contamination by S. subintegra, but not all of the diatom contamination. The population of the latter was reduced by using physical treatment and by washing with detergent and distilled water and then exterminated by using germanium dioxide (0.003 mg/L). Employing this protocol, unialgal cultures of H. musciformis could be established in approximately eight to ten weeks.
机译：海藻中的去污协议是实验室培养中生态生理研究的重要工具。这些协议包括必须针对每种物种进行调整的一组程序以及物理和化学处理。因此，外植体大小以及物理处理（刷毛和切割）和化学处理（次氯酸钠，去污剂，海水，蒸馏水，二氧化锗）的组合对获得两个Mypnea musciformis色素状形态的单藻培养的影响为调查。发现从田间运到实验室的长度为50毫米的mm节段，比长度为7毫米的节段更健康，对附生植物的敏感度更低。收集的材料的表面被硅藻污染，附着力很弱，以及由于强力附着的Sahlingia亚整合体造成的表面污染。物理和化学处理的最有效组合是外植体刷，切割和用洗涤剂洗涤。这种结合消除了亚整合链球菌的污染，但没有消除所有的硅藻污染。通过物理处理并通过用洗涤剂和蒸馏水洗涤减少了后者的数量，然后通过使用二氧化锗（0.003 mg / L）消除了后者。采用该方案，可以在大约八至十周内建立起莫氏形un的单藻培养物。

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