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首页> 外文期刊>Revista Brasileira de Hematologia e Hemoterapia >Quantification of CD8+CD38+ T lymphocytes by flow cytometry does not represent a good biomarker to monitor the reactivation of cytomegalovirus infection after allogeneic hematopoietic stem cell transplantation
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Quantification of CD8+CD38+ T lymphocytes by flow cytometry does not represent a good biomarker to monitor the reactivation of cytomegalovirus infection after allogeneic hematopoietic stem cell transplantation

机译：通过流式细胞术定量CD8 + CD38 + T淋巴细胞不能代表监测同种异体造血干细胞移植后巨细胞病毒感染的重新激活的良好生物标志物

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BACKGROUND: Infection/reactivation of cytomegalovirus is a major cause of morbidity and mortality in immunocompromised transplant patients. It has already been observed in kidney and liver transplantation patients that cytomegalovirus disease is accompanied by significant increases in circulating CD8+CD38+ T lymphocytes. There are no reports that study CD8+CD38+ T lymphocytes to monitor/diagnose cytomegalovirus disease in hematopoietic stem cell transplantation patients. OBJECTIVE: The aim of this study was to evaluate some cellular activation markers on circulating mononuclear cells (CD38 and HLA-DR) in patients submitted to hematopoietic stem cell transplantation and to establish any correlation with cytomegalovirus disease as diagnosed by antigenemia. METHODS: Blood samples of 15 transplant patients were analyzed by flow cytometry using anti-CD3, anti-CD4, anti-CD8, anti-CD38, CD16, CD56 and anti-HLA-DR monoclonal antibodies and the results were evaluated in respect to cytomegalovirus antigenemia measured by indirect immunofluorescence. Minitab for Windows was used for statistical analysis and a p-value < 0.05 was considered significant. RESULTS: Patients with positive antigenemia did not show any significant increase in the percentages of cells expressing the CD38 or HLADR activation markers when compared to patients with negative antigenemia. On the contrary, all patients showed high percentages of these cells independent of the presence of cytomegalovirus disease. CONCLUSIONS: This study suggests that the investigation of these lymphocyte sub-populations in patients submitted to hematopoietic stem cell transplantation does not seem to contribute to the early identification of cytomegalovirus disease.
机译：背景：巨细胞病毒的感染/再激活是免疫受损的移植患者发病和死亡的主要原因。已经在肾脏和肝脏移植患者中观察到巨细胞病毒病伴随着循环CD8 + CD38 + T淋巴细胞的显着增加。没有报道研究CD8 + CD38 + T淋巴细胞来监测/诊断造血干细胞移植患者的巨细胞病毒病。目的：本研究的目的是评估接受造血干细胞移植的患者循环单核细胞（CD38和HLA-DR）的某些细胞活化标记，并确定与抗原血症所诊断的巨细胞病毒病的任何相关性。方法：使用抗CD3，抗CD4，抗CD8，抗CD38，CD16，CD56和抗HLA-DR单克隆抗体，通过流式细胞术对15例移植患者的血样进行分析，并对巨细胞病毒的结果进行评估通过间接免疫荧光测定的抗原血症。用于Windows的Minitab用于统计分析，p值<0.05被认为是显着的。结果：与阴性抗原血症的患者相比，抗原血症阳性的患者表达CD38或HLADR激活标志物的细胞百分比没有显着增加。相反，所有患者均显示出高百分比的这些细胞，而与巨细胞病毒病的存在无关。结论：这项研究表明，对接受造血干细胞移植的患者中这些淋巴细胞亚群的调查似乎无助于早期识别巨细胞病毒病。

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