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首页> 外文期刊>Revista Brasileira de Ortopedia >Intra-articular viscosupplementation of hyaluronic acids in an experimental osteoarthritis model
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Intra-articular viscosupplementation of hyaluronic acids in an experimental osteoarthritis model

机译：在实验性骨关节炎模型中透明质酸的关节腔内黏膜补充

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Objective To analyze, from the immunohistochemical perspective, the effects of hyaluronic acid of different molecular weights in an experimental model of osteoarthritis in rabbits. Methods Forty-four male California rabbits were randomly assigned to three different groups (PR, S, and P) and submitted to the resection of the anterior cruciate ligament of the right knee. Three weeks after the surgical procedure, three intra-articular weekly injections were carried out with low-molecular-weight native hyaluronic acid (Hyalgan ? ) to PR group, high molecular weight branched chain hyaluronic acid (Synvisc ? ) to group S, and saline solution 0.9% to group P. All animals were sacrificed 12 weeks after the surgical procedure, and the tibial plateaus of the infiltrated knees were then dissected. Histological sections of cartilage from the tibial plateau support areas were stained with immunohistochemical markers in order to investigate the amount of metalloproteases (MMPs 3 and 13) and their inhibitors (TIMPs 1 and 3). The staining intensity was quantified on a Zeiss Imager.Z2 Metasystems microscope and analyzed by Metafer4 Msearch software. Results The chondroprotective effect of the hyaluronic acids used in the study was demonstrated when compared to the control group. However, the comparison between them presented no significant statistical difference regarding chondroprotection. Conclusion The injection of saline solution demonstrated signs of OA development, while adding native hyaluronic acid of low molecular weight (Hyalgan ? ) and hyaluronic acid of high molecular weight (Synvisc ? ) protected the articular cartilage in this model of OA.
机译：目的从免疫组化的角度分析不同分子量的透明质酸在兔骨关节炎实验模型中的作用。方法将44只雄性加利福尼亚兔随机分为三组（PR，S和P），并进行右膝前交叉韧带切除术。手术后三周，每周进行三次关节内注射，分别向PR组低分子量天然透明质酸（Hyalgan？），向S组高分子量支链透明质酸（Synvisc？）和生理盐水。溶液0.9％加入P组。在手术后12周处死所有动物，然后解剖浸润膝盖的胫骨平台。为了研究金属蛋白酶（MMP 3和13）及其抑制剂（TIMP 1和3）的数量，使用免疫组织化学标记对来自胫骨平台支持区的软骨的组织学切片进行了染色。在Zeiss Imager.Z2 Metasystems显微镜上定量染色强度，并通过Metafer4 Msearch软件进行分析。结果与对照组相比，该研究中所用的透明质酸的软骨保护作用得到了证明。但是，它们之间的比较没有显示出有关软骨保护的显着统计学差异。结论注射盐溶液显示出OA的发展迹象，而在这种OA模型中，加入低分子量的天然透明质酸（Hyalgan？）和高分子量的透明质酸（Synvisc？）保护了关节软骨。

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