...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista de Ciências Farmacêuticas Básica e Aplicada >Anion exchange resin as support for invertase immobilization
【24h】

Anion exchange resin as support for invertase immobilization

机译：阴离子交换树脂作为固定化转化酶的载体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The invertase (EC 3.2.1.26) from Saccharomyces cerevisiae was employed as a model enzyme in the evaluation of the adsorption capacity of DOWEX-1X8-50?, a basic anion exchange resin, when used as support in enzyme immobilization. By mixing 100mg of resin with 27mg of invertase (pI = 4.0) in buffer solution (pH 4.6, 25°C), stirred at 100rpm, an adsorption of 93% was achieved. The activities (1U = amount of enzyme forming 1mg reducing sugars/min) of soluble and insoluble invertase were 0.084 U/mgE and 0.075 U/mgE, respectively, giving an immobilization coefficient of 90.4%. The immobilized invertase had a higher thermal stability than the soluble form. The highest activity was observed at pH 4.5 in both forms of the enzyme, whereas the pH stability ranges for soluble and insoluble invertase were 3.5-5.0 and 4.5-5.5, respectively. The kinetic constants for soluble invertase were KM = 18.3 mM and Vmax = 0.084 U/mgE, and for the insoluble form, KM = 29.1 mM and Vmax = 0.075 U/mgE. The resin tested adsorbed the invertase very well, provided the enzyme molecule had a net negative charge, i.e., the immobilization and reaction procedures had to be carried out at pH > pI.
机译：当评价碱性阴离子交换树脂DOWEX-1X8-50？的吸附能力用作酶固定化的载体时，将酿酒酵母的转化酶（EC 3.2.1.26）用作模型酶。通过在缓冲溶液（pH 4.6，25°C）中将100mg树脂与27mg转化酶（pI = 4.0）混合，以100rpm的速度搅拌，吸附率为93％。可溶性和不溶性转化酶的活性（1U =形成1mg还原糖/分钟的酶量）分别为0.084 U / mgE和0.075 U / mgE，固定系数为90.4％。固定化的转化酶具有比可溶性形式更高的热稳定性。两种形式的酶在pH 4.5时均观察到最高活性，而可溶性和不溶性转化酶的pH稳定范围分别为3.5-5.0和4.5-5.5。可溶性转化酶的动力学常数为KM = 18.3 mM和Vmax = 0.084 U / mgE，不溶形式的KM = 29.1 mM和Vmax = 0.075 U / mgE。如果酶分子具有净负电荷，则被测试的树脂很好地吸附了转化酶，即，固定化和反应程序必须在pH> pI下进行。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号