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首页> 外文期刊>Revista Cubana de plantas medicinales >Validation of a High Performance Liquid Chromatography technique for determination of mangiferin content in Mangifera indica L. leaves
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Validation of a High Performance Liquid Chromatography technique for determination of mangiferin content in Mangifera indica L. leaves

机译：高效液相色谱法测定芒果叶中芒果苷含量的验证

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摘要

Introduction: Mangiferin is a natural glycosylxanthone present in various parts of the tree Mangifera indica L. which is attributed a wide range of pharmacological properties. Objective: establish a method to determine the content of mangiferin in Mangifera Indica L. leaves by high performance liquid chromatography. Methods: leaves were dried and pulverized before quantification of their metabolite content. Separation was performed using a Luna RP-18 chromatographic column (5 µm) (250 x 4mm) with a UV detector at 254n and a mobile phase A gradient composed of acetic acid 0.2 %: acetonitrile (85:15), and a phase B mobile: acetonitrile and its quantification vs. a reference sample applying the external standard method. Results: the regression curve equation for mangiferin was Y=3.5561X +6536.55(r=0.999). Recovery was 99.41 % with a variation coefficient below 2 %. Detection and quantification limits were 0.0009 and 0.001, respectively. No statistically significant differences were found between the determinations performed on different days by different analysts. Conclusion: the method is simple and reliable, and may be used for quality control and stability studies about Mangifera Indica L. leaves.
机译：简介：芒果苷是一种天然的糖基various吨酮，存在于芒果树的各个部位，具有广泛的药理特性。目的：建立高效液相色谱法测定芒果叶中芒果苷的含量的方法。方法：将叶片干燥并粉碎，然后定量其代谢物含量。使用Luna RP-18色谱柱（5 µm）（250 x 4mm），在254n处有UV检测器和流动相A进行分离，流动相A的梯度为乙酸0.2％：乙腈（85:15），B相移动：乙腈及其定量与使用外标方法的参考样品相比。结果：芒果苷的回归曲线方程为Y = 3.5561X +6536.55（r = 0.999）。回收率为99.41％，变异系数低于2％。检测限和定量限分别为0.0009和0.001。不同分析师在不同天进行的测定之间没有发现统计学上的显着差异。结论：该方法简便可靠，可用于芒果叶的质量控制和稳定性研究。

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