• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista de salud animal >Strains of Lactobacillus spp. with probiotic capacity isolated from the intestinal tract of calves
【24h】

Strains of Lactobacillus spp. with probiotic capacity isolated from the intestinal tract of calves

机译：乳酸杆菌属菌株。从小牛肠道分离出的益生菌

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Seventeen strains of Lactobacillus spp. isolated from the intestinal tract of neonatal calves were characterized in vitro to select the best ones as probiotic candidates and to study them in vivo. All the isolates were tested for catalase, Gram stained, and their cultural characteristics studied for their preliminary identification. Their probiotic capacity, such as growth at different temperatures (30, 37, and 45°C), pH (2.5, 3.4, 5.4, 6.4), and their capacity to acidify the culture medium, were assessed in vitro. Ten strains of Lactobacillus spp. growing under all the conditions tested were preselected, standing out growths of viable cells of 6,7-10 log CFU/ml that reduced pH values to 3.2-4.0. Then, their functional characteristics such as tolerance to artificial gastric juice and to bile salts (0.3% Oxgall), and their microbial antagonism activity against four pathogens of veterinary interest were evaluated. The result obtained showed probiotic potential in four of the Lactobacillus spp. strains tested for their resistance to treatment with bile salts until 24 hours and the microbial growth of 3.52-6.5 log CFU/ml with artificial gastric juice observed after 90 minutes; they also inhibited the growth of Salmonella sp., Staphylococcus aureus, Escherichia coli and Listeria monocytogenes. The profile of susceptibility to 13 antimicrobials was evaluated, and most of the strains revealed resistance to three of them.
机译：乳酸杆菌属的十七株。从新生儿小牛肠道分离出的细菌在体外进行表征，以选择最佳的作为益生菌候选物并进行体内研究。测试所有分离株的过氧化氢酶，革兰氏染色，并研究其培养特性以进行初步鉴定。在体外评估了它们的益生菌能力，例如在不同温度（30、37和45°C）下的生长，pH（2.5、3.4、5.4、6.4）及其酸化培养基的能力。乳酸杆菌属的十株。预先选择了在所有测试条件下均能生长的细菌，其存活细胞的生长速度高达6,7-10 log CFU / ml，可将pH值降至3.2-4.0。然后，评估了它们的功能特性，如对人造胃液和胆汁盐（0.3％Oxgall）的耐受性，以及它们对四种感兴趣的兽医病原体的微生物拮抗活性。获得的结果显示了四个乳酸杆菌属中的益生菌潜力。测试菌株直至24小时对胆汁盐处理的抗性，并在90分钟后观察到用人工胃液的微生物生长为3.52-6.5 log CFU / ml;它们还抑制沙门氏菌，金黄色葡萄球菌，大肠杆菌和李斯特菌的生长。评估了对13种抗菌药的敏感性，大多数菌株显示出对其中三种的耐药性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号