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首页> 外文期刊>Revista de salud animal >Effect of sperm separation techniques on the viability and acrosome reaction of frozen-thawed ovine spermatozoids
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Effect of sperm separation techniques on the viability and acrosome reaction of frozen-thawed ovine spermatozoids

机译：精子分离技术对冻融绵羊精子活力和顶体反应的影响

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The effectiveness of four sperm separation techniques on thawed ovine semen was evaluated. A hundred and sixty straws with 0.5 ml of semen with a concentration of 200x106/ml were used. In each work session, four straws were thawed at 37.5ºC for 45 sec mixing their contents. Then, 200 µl were taken to perform one of the following techniques: Swim-up, Percoll, filtering through glass fiber or filtering through Sephadex. Increased progressive motility of spermatozoids was obtained through swim-up, glass fiber and Sephadex (56.1%, 66.4%, and 56.8%, respectively), which was significantly different (p0.05) in those recovered from glass fiber. The numbers of live spematozoids with acrosome reaction were high with all the separation techniques, with a significant difference (p0.05) in those obtained from swim-up, glass fiber and Sephadex, whose values were higher than 50.0%. The numbers of dead spermatozoids, with and without acrosome reaction, were low in all the techniques, with the exception of those obtained by Percoll, which was significantly different (p0.05) to the control, excepting those obtained by Sephadex and swim-up when the dead ones with acrosome reaction were evaluated. When evaluating live spermatozoids without acrosome reaction, a low value of this indicator was found in all the treatments (13.1, 9.1, 9.3 and 10.8%, swim-up, Percoll, glass fiber and Sephadex, respectively. The sperm viability evaluated with the hypo-osmotic test and propidium iodide concluded that a higher percentage of live spermatozoids were obtained when they were filtered with glass fiber (73.0% and 68.3%, hypo-osmotic test and propidium iodide, respectively); no significant differences (p0.05) were observed between these two last test.
机译：评价了四种精子分离技术对解冻的绵羊精液的有效性。使用160根含0.5 ml精液的吸管，浓度为200x106 / ml。在每个工作阶段中，将4根吸管在37.5ºC融化45秒，融化其中的内容物。然后，取200 µl进行以下技术之一：游泳，Percoll，通过玻璃纤维过滤或通过Sephadex过滤。通过游泳，玻璃纤维和Sephadex（分别为56.1％，66.4％和56.8％）获得了增加的精子进行性运动能力，这与从玻璃纤维中回收的精子有显着差异（p <0.05）。在所有分离技术中，具有顶体反应的活体水肿数量都很高，其中从游泳液，玻璃纤维和Sephadex中获得的活体水肿率均高于50.0％，差异具有显着性（p <0.05）。在所有技术中，有和没有顶体反应的死精子数量都很少，除了通过Percoll获得的精子数量与对照组相比有显着差异（p <0.05），除了通过Sephadex和游泳获得的精子数量。对死者顶体反应进行评估。当评估没有顶体反应的活精子时，在所有治疗（游泳，Percoll，玻璃纤维和Sephadex处理）中，该指标的值均较低（分别为13.1、9.1、9.3和10.8％）。渗透试验和碘化丙锭的结论是，用玻璃纤维过滤时，获得的活精子比例更高（分别为73.0％和68.3％，低渗透试验和碘化丙啶）;无显着差异（p <0.05）在最后两个测试之间观察到。

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