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Identification of genes codifying for VirB9, VirB10, conjugal transfer protein and elongation factor Tu of an Anaplasma marginale Cuban isolate

机译:鉴定编码古巴无形体分离株的VirB9,VirB10,接合转移蛋白和延伸因子Tu的基因

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From the sequencing of the entire genome of Anaplasma marginale isolates, genes encoding membrane proteins of this hemoparasite have been identified, as well as novel immunogens as potential vaccine candidates. However, little is known about the genetic variability of genes encoding membrane proteins of Anaplasma marginale isolates. The aim of this study was to determine the degree of conservation of the genes encoding the VirB9, VirB10 proteins, conjugal transfer protein (CTP) and the elongation factor ( Ef- Tu) in the nucleotide sequence of a Cuban Anaplasma marginale isolate and from the deduced amino acid sequence and compare them with those described for Florida and Saint Maries Anaplasma marginale isolates and Anaplasma (centrale) marginale from Israel . The gene and deduced amino acid sequences showed high degree of identity with the sequences described for other isolates of Anaplasma marginale.
机译:通过对无性无浆膜分离物的整个基因组进行测序,已经鉴定出编码该血寄生虫的膜蛋白的基因,以及新的免疫原作为潜在的候选疫苗。然而,鲜为人知无浆膜分离株的膜蛋白编码基因的遗传变异知之甚少。本研究的目的是确定古巴无缘种分离株核苷酸序列中编码VirB9,VirB10蛋白,接合转移蛋白(CTP)和延伸因子(Ef-Tu)的基因的保守程度。推导得出的氨基酸序列,并将其与针对以色列的佛罗里达和圣马里氏无核小种分离物和以色列无核无性种(中央)的描述进行比较。该基因和推导的氨基酸序列显示出与其他无缘无浆膜分离物所描述的序列高度相同。

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