• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Revista de salud animal >Cryopreservation and storage of Mycoplasma spp.
【24h】

Cryopreservation and storage of Mycoplasma spp.

机译：支原体的低温保存和保存。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cryopreservation has as a principle to maintain cell viability and functionality at low temperatures; thus, to understand and properly implement cryopreservation of biological material is essential in laboratories and cell banks. In the case of mycoplasmas, maintenance of viable cultures is not only an operational issue, but also an essential factor in the good performance of the laboratory work. Therefore, this study aimed to assess the viability of mycoplasma strains preserved by freezing, in different matrices and several cryopreserving agents. For the study, it was used a culture of Mycoplasma hyorhinis (selected as type strain) with 5,1x108UFC/ml, which was inoculated into cell culture matrices and ascetic liquid. Both types of samples were stored at -70ºC and -20ºC by using or not cryopreserving agents; in this case, 5% glycerol and 2.5% dimethylsulfoxide were used. At 3, 30, 60 and 90 days postfreezing, mycoplasma viability was checked by growing them in liquid and solid Hayflick's media. As a result, it was found that in the matrices frozen at -20ºC and -70ºC and cryopreserving, Mycoplasma hyorhinis remained viable for 90 days, but not in the matrices containing no cryopreserving, where the behavior was variable depending on the temperature. It was concluded that the addition of cryopreserving agents (5% glycerol or 2.5% dimethylsulfoxide) preserved the viability of mycoplasmas in the matrices studied during freezing and subsequent storage; also the temperature of -20ºC can be used by those laboratories lacking equipment with -70ºC to store samples for three months, period of time designed for interlaboratory studies.
机译：低温保存的原则是在低温下保持细胞活力和功能。因此，在实验室和细胞库中，了解并正确实施生物材料的低温保存至关重要。就支原体而言，维持可行的培养不仅是操作上的问题，而且还是实验室工作良好表现的重要因素。因此，本研究旨在评估在不同基质和几种冷冻保存剂中冷冻保存的支原体菌株的活力。为了进行研究，将其以5,1x108UFC / ml的猪支原体（选择为类型菌株）的培养物接种到细胞培养基质和苦液中。两种类型的样品都使用或不使用低温保存剂保存在-70ºC和-20ºC下。在这种情况下，使用5％的甘油和2.5％的二甲基亚砜。冷冻后3、30、60和90天，通过在液体和固体Hayflick培养基中生长支原体来检查其活力。结果发现，在-20°C和-70°C冷冻并冷冻保存的基质中，Mycoplasma hyorhinis仍然可以存活90天，而在没有冷冻保存的基质中则没有，其行为随温度而变化。结论是，冷冻保存剂（5％的甘油或2.5％的二甲基亚砜）的加入在冷冻和随后的保存过程中保持了支原体在所研究基质中的活力。那些缺乏配备-70ºC设备的实验室也可以使用-20ºC的温度来将样品存储三个月，这段时间是为实验室间研究而设计的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号