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Prevalence of Mollicutes in Cell Cultures: experience in Cuba

机译:细胞培养中毛囊炎的流行:古巴的经验

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Different mollicutes species of the Mycoplasma and Acholeplasma genera can contaminate the cell cultures and raw materials commonly used in the manufacture of a variety of biological and therapeutical products. In this study, the presence of mollicutes was detected in 19 of 50 cell culture samples analyzed using PCR and microbiological cultures. The species most frequently detected was M. fermentans (31.5%), followed by A. laidlawii (26.3%), M. orale (15.7%), M. hyorhinis (15.7%), and M. salivarium (10, 5%). A. laidlawii was present as the infecting agent only in 10.5% of the samples. These results showed the main species of contaminants, which suggested mollicutes multiple origin in the source of infection.
机译:支原体和无形体属的不同种类的分子会污染通常用于生产各种生物和治疗产品的细胞培养物和原材料。在这项研究中,在使用PCR和微生物培养物分析的50个细胞培养物中,有19个检测到了软体动物。最常检测到的物种是发酵发酵单胞菌(31.5%),其次是A. laylawii(26.3%),M。orale(15.7%),M。hyorhinis(15.7%)和S.salivarium(10,5%)。 。仅有10.5%的样品中存在莱德劳伊菌作为感染因子。这些结果表明了主要的污染物种类,这表明在感染源中存在多种分子。

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