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首页> 外文期刊>Revista de salud animal >EVALUATION OF METHODS FOR THE PRIMARY ISOLATION OF Brucella melitensis IN GOAT MILK
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EVALUATION OF METHODS FOR THE PRIMARY ISOLATION OF Brucella melitensis IN GOAT MILK

机译：山羊奶中布鲁氏菌初次分离方法的评价

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In order to evaluate different methods for the primary isolation of Brucella melitensis in goat milk from infected herds, Ver., 45 female adult goats were randomly selected trough the following criteria: 30 previously vaccinated with Brucella melitensis Rev-1 strain, and 15 never vaccinated. Sample size was estimated by a probabilistic model, with unknown population and 35% known prevalence rate (n = 1 - p / pv); where n corresponds to sample size, p for prevalence rate and v variation coefficient (0.05). All goats selected were sampled three times within a 30 day interval to guarantee Brucella spp. milk shedding. Milk samples were aseptically collected into sterile glass tubes from half udder and were refrigerated at 4°C until they were laboratory processed into the next 24 hours. Milk samples were 10,000 X g centrifuged during 20 minutes, in a refrigerated centrifuge at 4oC. Farrell and Tripticase Soy Agar (TSA) media, were the isolation culture media selected and both were enriched with bovine fresh serum during their formulation. Both isolation culture media selected were inoculated by duplicate with fat and sediment from centrifuged milk as it is established in the Mexican regulations. The results obtained were statistically evaluated by Chi square (c2). A half of both inoculated isolation culture media was incubated under 5 to 10% carbonic dioxide atmosphere and the other half in aerobic one. From 76% of 135 milk samples obtained, only Brucella melitensis biovar - 1 was isolated using both culture media; however, the majority of isolated field strains were recovered from Farrell culture medium (p0.05); from milk fat 57% of isolated strains and from milk sediment 67%. Also, the best Brucella melitensis biovar - 1 development (p0.05) from Farrell culture medium was observed by aerobic atmosphere incubation. It is conclusive that Farrell culture medium is more effective than TSA medium for that purpose, both from the fat and sediment of milk, likewise incubation of the samples in aerobic atmosphere was better and there was more successful development of bacteria.
机译：为了评估从感染牛群中从牛奶中初步分离出布鲁氏菌的方法，Ver。通过以下标准随机选择了45只成年雌性山羊：30例先前接种过布鲁氏菌Rev-1株，而15从未接种过。样本量由概率模型估计，人口未知，已知患病率35％（n = 1-p / pv）;其中，n代表样本量，p代表患病率，v代表变异系数（0.05）。在30天的间隔内对所有选择的山羊进行3次采样，以确保布鲁氏菌属。牛奶脱落。从半乳中将牛奶样品无菌收集到无菌玻璃管中，并在4°C冷藏，直到将其实验室处理至接下来的24小时。将牛奶样品在4oC的冷冻离心机中于20分钟内离心10,000 X g。 Farrell和Tripticase大豆琼脂（TSA）培养基是选择的分离培养基，在配制过程中均富含牛新鲜血清。按照墨西哥法规的规定，选择的两种分离培养基均要一式两份接种离心牛奶中的脂肪和沉淀物。通过卡方（c2）对获得的结果进行统计学评估。将两种接种的分离培养基的一半在5至10％的二氧化碳气氛下孵育，另一半在有氧培养基中孵育。从两种牛奶中分离出的135份牛奶样品中，有76％仅分离了布鲁氏菌biovar-1。但是，大多数分离的田间菌株是从Farrell培养基中回收的（p <0.05）;来自57％分离菌株的乳脂和67％的牛奶沉淀物。另外，通过有氧气氛培养观察到最佳的法氏布鲁氏菌biovar-1发育（p <0.05）。可以肯定的是，Farrell培养基比TSA培养基更有效，无论是从牛奶中的脂肪还是在沉积物中，在有氧环境中样品的培养效果都更好，细菌的繁殖也更成功。

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