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首页> 外文期刊>Rice >Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice
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Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice

机译：赋予水稻广谱白叶枯病抗性的三个金字塔抗性基因育种系的开发及其分子分析

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BackgroundThe development of resistant cultivars has been the most effective and economical strategy to control bacterial leaf blight (BB) disease of rice caused by Xanthomonas oryzae pv. oryzae ( Xoo ). Molecular markers have made it possible to identify and pyramid valuable genes of agronomic importance in resistance rice breeding. In this study, three resistance genes ( Xa4 + xa5 + Xa21 ) were transferred from an indica donor (IRBB57), using a marker-assisted backcrossing (MAB) breeding strategy, into a BB-susceptible elite japonica rice cultivar, Mangeumbyeo, which is high yielding with good grain quality. ResultsOur analysis led to the development of three elite advanced backcross breeding lines (ABL) with three resistance genes by foreground and phenotypic selection in a japonica genetic background without linkage drag. The background genome recovery of the ABL expressed more than 92.1% using genome-wide SSR marker analysis. The pathogenicity assays of three resistance-gene-derived ABL were conducted under glasshouse conditions with the 18 isolates of Xoo prevalent in Korea. The ABL exhibited very small lesion lengths, indicating a hypersensitive reaction to all 18 isolates of Xoo , with agronomic and grain quality traits similar to those of the recurrent parent. Pyramiding the resistance genes Xa4, xa5 and Xa21 provided a higher resistance to Xoo than the introduction of the individual resistance genes. Additionally, the combination of two dominant and one recessive BB resistance gene did not express any negative effect on agronomic traits in the ABL. ConclusionsThe strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice. The resistance-gene-derived ABL have practical breeding value without a yield penalty by providing broad-spectrum resistance against most of the existing isolates of BB in South Korea and will have a high impact on the yield stability and sustainability of rice productivity.
机译：背景技术抗性品种的发展一直是控制稻米黄单胞菌（Xanthomonas oryzae pv）引起的水稻细菌性叶枯病（BB）病最有效，最经济的策略。稻（Xoo）。分子标记使人们有可能在抗性水稻育种中鉴定出具有重要农业价值的重要基因并将其金字塔化。在这项研究中，通过标记辅助回交（MAB）育种策略，将三个抗性基因（Xa4 + xa5 + Xa21）从in稻供体（IRBB57）转移至对BB敏感的粳稻品种Mangeumbyeo，高产，优质谷物。结果我们的分析导致在无连锁拖曳的粳稻遗传背景下通过前景和表型选择开发了具有三个抗性基因的三个优良的先进回交育种系（ABL）。使用全基因组SSR标记分析，ABL的背景基因组回收率显示超过92.1％。在温室条件下，对韩国流行的18种Xoo分离株进行了3种抗药源性ABL的致病性分析。 ABL表现出非常小的病灶长度，表明对Xoo的所有18种分离物都具有过敏反应，其农艺和谷物品质性状与轮回亲本相似。与引入单个抗性基因相比，使抗性基因Xa4，xa5和Xa21金字塔化可提供更高的Xoo抗性。另外，两个显性和一个隐性BB抗性基因的组合对ABL中的农艺性状没有表达任何负面影响。结论同时前景和表型选择引入多个R基因的策略对于降低分离具有目标抗性基因的理想重组子的成本和所需时间非常有用。通过对韩国现有的大多数BB分离株提供广谱抗性，源自抗性基因的ABL具有实用的育种价值，不会造成产量损失，并且将对水稻的产量稳定性和可持续性产生重大影响。

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