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首页> 外文期刊>Romanian Journal of Biophysics >THE INTERACTION OF AMINOGLYCOSIDES WITH ENDOTHELIAL CELLS INFECTED BY PSEUDOMONAS AERUGINOSA
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THE INTERACTION OF AMINOGLYCOSIDES WITH ENDOTHELIAL CELLS INFECTED BY PSEUDOMONAS AERUGINOSA

机译：氨糖苷与铜绿假单胞菌感染的内皮细胞的相互作用

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The membrane organization of cultured human endothelial cells (EA.hy926) infected with Pseudomonas aeruginosa (PS) was studied in the temperature range 15–37 oC by using fluorescent depolarization and generalized polarization (GP) measurements on the cells suspensions labeled with TMA-DPH and laurdan, respectively. The opportunistic pathogen Pseudomonas aeruginosa was chosen since this pathogen is the etiologic agent of resistant and recurrent pulmonary infections in cystic fibrosis (CF) patients, who require sustained treatment with antibiotics. The effect of several aminoglycoside antibiotics – gentamicin, amikacin and kanamycin – on membrane organization of infected cultured cells was recorded. The measurements results showed that the presence of aminoglycosides cations induces a rigidity of the infected cell membrane, gentamicin being the most efficient in this respect. This effect is temperature sensitive, being much more pronounced at temperatures close to physiological range. The above information may be of use in determining the treatment regimen of a CF patient who has recurrent infections and requires antibiotics treatment for a longer time.
机译：在15-37 oC的温度范围内，使用荧光去极化和广义极化（GP）测量对TMA-DPH标记的细胞悬液进行了研究，研究了感染铜绿假单胞菌（PS）的人类内皮细胞（EA.hy926）的膜组织和劳丹，分别。选择机会性病原体铜绿假单胞菌是因为该病原体是需要持续用抗生素治疗的囊性纤维化（CF）患者的耐药性和复发性肺部感染的病原体。记录了几种氨基糖苷类抗生素（庆大霉素，丁胺卡那霉素和卡那霉素）对感染的培养细胞的膜组织的影响。测量结果表明，氨基糖苷类阳离子的存在会诱导被感染细胞膜的刚度，庆大霉素在这方面是最有效的。该效应对温度敏感，在接近生理范围的温度下更为明显。以上信息可用于确定患有反复感染且需要较长时间进行抗生素治疗的CF患者的治疗方案。

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