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首页> 外文期刊>Romanian Biotechnology Letters >A Comprehensive Approach to Utilize an Agricultural Pea peel (Pisum sativum) Waste as a Potential Source for Bio-ethanol Production
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A Comprehensive Approach to Utilize an Agricultural Pea peel (Pisum sativum) Waste as a Potential Source for Bio-ethanol Production

机译：利用农用豌豆皮（Pisum sativum）废料作为生物乙醇生产的潜在来源的综合方法

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In the present study, a Central Composite Design (CCD) from Response Surface Methodology (RSM) was adopted to produce bio-ethanol using pre-treated pea peel (Pisum sativum). With an aim to achieve optimal yield the concentration of acid load; 1.0, 1.5 and 2.0%, treatment time; 15, 30 and 45 min, and temperature in the range of 100, 110 and 120 °C was selected. Solid P. sativum loading of 10% (w/v) was selected during pre-treatment. After dilute sulfuric acid pre-treatment maximum increase in glucose content (35.97%) of pea peel was achieved at their optimized conditions (1.80%, 30min. and 120°C). Maximal yield of glucose (after 72 h) from pea peel after dilute sulfuric acid pre- treatment and enzymatic saccharification (50 °C, pH 4.8 and 120 rpm) by using an enzyme loading of 0.11 g celluclast 1.5 g/L cellulase and 0.05 g cellubiase/g was 17.72 g/L. Fermentation, the last and important step of bio-ethanol production was carried out using fermentative fungal S. bayanus and F. oxysporum. The bio-ethanol yield from pre-treated enzyme saccharified pea peel solid fraction (50 g) by S. bayanus at pH 4.8, and 25 °C in 72h was 0.37 g/g of available glucose and 0.16 g/g of dry pea peel whereas in case of F. oxysporum under the same conditions except at pH 6, the ethanol yield was 0.29 g/g of available glucose and 0.13 g/g of dry matter. From these results we come to know that pea peel can also prove a better candidate for bio-ethanol production on large scale.
机译：在本研究中，采用响应表面方法学（RSM）的中央复合设计（CCD）通过预处理的豌豆皮（Pisum sativum）生产生物乙醇。为了达到最佳产量，酸负荷的浓度; 1.0、1.5和2.0％的治疗时间;选择15、30和45分钟，温度范围为100、110和120°C。在预处理过程中选择了10％（w / v）的固态体育葡萄负载量。稀硫酸预处理后，在其最佳条件（1.80％，30分钟和120°C）下，豌豆皮的葡萄糖含量最大增加（35.97％）。稀硫酸预处理和酶促糖化（50°C，pH 4.8和120 rpm）（使用0.11 g纤维素酶1.5 g / L纤维素酶和0.05 g酶）后豌豆皮的最大葡萄糖产量（72小时后）纤维素酶/ g为17.72g / L。发酵，生物乙醇生产的最后也是重要步骤是使用发酵真菌巴彦链霉菌和尖孢镰刀菌进行的。在pH 4.8、25°C和72 h下，由巴氏链霉菌预处理的酶糖化豌豆皮固体部分（50 g）的生物乙醇产量为0.37 g / g可用葡萄糖和0.16 g / g干豌豆皮而在相同条件下（除pH值为6时），葡萄球菌的乙醇产量为0.29 g / g有效葡萄糖和0.13 g / g干物质。从这些结果中我们知道，豌豆皮也可以证明是大规模生产生物乙醇的更好候选者。

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