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首页> 外文期刊>Romanian Biotechnology Letters >The effect of some insecticides on soil microorganisms based on enzymatic and bacteriological analyses
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The effect of some insecticides on soil microorganisms based on enzymatic and bacteriological analyses

机译：基于酶和细菌学分析的某些杀虫剂对土壤微生物的影响

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摘要

The use of insecticides in crops is meant to protect plants against harmful insects and increases crop yields. At soil level, insecticides can influence physico-chemical proprieties but especially the biological proprieties of soil, influencing the number and metabolic activity of soil microbial communities. The analiyzed soil samples come from experimental plots where cypermethrin and thiamethoxam were applied in field conditions. Biochemical analyses consisted in the determination of the dehydrogenase, urease, catalase and phosphatase enzymatic activities and bacteriological analyses consisted in the determination of the number of bacteria belonging to the ecophysiological groups involved in the nitrogen cycle in soil. Cypermethrin and thiamethoxam showed an inhibitory effect on metabolic processes in soil, the registered values for dehydrogenase, urease, catalase and phosphatase enzymatic activities being statistically significantly lower than in the untreated sample. The pH and humidity influenced the enzymatic activities, positive correlations being established with dehydrogenase, catalase and urease activities and negative correlations with phosphatase activity. The ecophysiological groups of bacteria anlyzed (ammonifying bacteria, nitrifying bacteria and denitrifying bacteria) showed statistically significant decreases in experimental variants treated with cypermethrin and thiamethoxam compared to the untreated sample. pH and humidity showed positive or negative correlations according to the ecophysiological group analyzed.
机译：在农作物中使用杀虫剂旨在保护植物免受有害昆虫的侵害，并提高农作物的产量。在土壤水平上，杀虫剂会影响土壤的理化特性，但尤其会影响土壤的生物学特性，从而影响土壤微生物群落的数量和代谢活性。被分析的土壤样品来自实验田，在现场条件下使用了氯氰菊酯和噻虫嗪。生化分析包括确定脱氢酶，脲酶，过氧化氢酶和磷酸酶的酶活性，细菌学分析包括确定属于土壤氮循环的生态生理学细菌的数量。氯氰菊酯和噻虫嗪对土壤中的代谢过程具有抑制作用，脱氢酶，脲酶，过氧化氢酶和磷酸酶的酶活性的注册值在统计学上显着低于未处理的样品。 pH和湿度影响酶的活性，与脱氢酶，过氧化氢酶和脲酶的活性呈正相关，与磷酸酶的活性呈负相关。与未处理的样品相比，用氯氰菊酯和噻虫嗪处理的实验变体中，被分析的细菌（氨化细菌，硝化细菌和反硝化细菌）的生态生理组显示出统计学上显着的下降。根据所分析的生态生理组，pH和湿度呈正相关或负相关。

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