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BIOPHYSICAL MEASUREMENTS OF LEAD IN SOME BIOINDICATOR PLANTS

机译:某些生物指示剂植物中铅的生物物理测量

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Heavy metal (lead) concentrations in two marsh plants (Phragmites australis and Cyperus rotundus) and one free floating hydrophyte (Eichhornia crassipes) growing near metal smelters (lead pollutant area) were investigated. The shoots of the investigated plants were analyzed for lead content by flame atomic absorption spectrometry. The data indicated high lead levels due to the influence of urban and industrial effluents. This showed that the target plants could be considered as lead pollution biosensors. Scanning electron microscopy (SEM) and positron annihilation lifetimes (PAL) technique were used to ascertain the localization of lead in the living tissues. Cross-sections of plant organs were examined using SEM attached with an Energy Dispersed X-ray (EDX) unit for elemental analysis of the formed crystals that appeared abundant in the aerenchyma tissues of the polluted plants. PAL data reflected the correlation between the fraction of free volume hole and lead concentration; higher lead accumulation gives lower fraction of free volume hole. The results demonstrate the extreme sensitivity of the PAL technique towards structural changes occurring in polluted plants.
机译:研究了在金属冶炼厂(铅污染区)附近生长的两座沼泽植物(芦苇和香附子)和一棵游离浮游水生植物(Eichhornia crassipes)中的重金属(铅)浓度。通过火焰原子吸收光谱法分析所研究植物的茎中的铅含量。数据表明,由于城市和工业废水的影响,铅含量很高。这表明目标植物可被视为铅污染生物传感器。使用扫描电子显微镜(SEM)和正电子an灭寿命(PAL)技术确定铅在活组织中的定位。使用附有能量分散X射线(EDX)单元的SEM检查植物器官的横截面,以对形成的晶体进行元素分析,该晶体在受污染植物的气孔组织中似乎很丰富。 PAL数据反映了自由体积空穴的分数与铅浓度之间的相关性;较高的铅累积量会降低自由体积孔的比例。结果表明,PAL技术对受污染植物中发生的结构变化具有极高的敏感性。

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