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首页> 外文期刊>Romanian Journal of Biophysics >BASIC FEATURES OF SENSORY NEURONS FROM DORSAL ROOT GANGLIA IN TCR-HA+/–/RIP-HA+/– MICE
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BASIC FEATURES OF SENSORY NEURONS FROM DORSAL ROOT GANGLIA IN TCR-HA+/–/RIP-HA+/– MICE

机译：TCR-HA + / – / RIP-HA +/–小鼠背根神经节感觉神经元的基本特征

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Despite the increased prevalence of peripheral diabetic neuropathy, some of its mechanisms are still unknown. In our study, we have used a double transgenic mice (dTg) model, TCR-HA+/–/RIP-HA+/–, which develops a fulminate type of insulino-dependent diabetes. The goal of our study was to reveal the molecular mechanisms of diabetic neuropathy in neuronal primary cultures. The presence of the transgenes, that are responsible for the etiophatogenesis of type I diabetes is determined by the PCR technique. Blood glucose, insulin level and corporal weight are monitored after 4 weeks of life. Neuronal primary cultures are dissociated from dorsal root ganglia. Electrophysiological recordings of these neurons are performed by patch-clamp technique, whole-cell configuration. In our study we have recorded the characteristics of the action potential and the hyperpolarization activated currents in sensory neurons. Significant differences were evidenced between the electrophysiological profiles of the sensory neurons from dorsal root ganglia prelevated from TCR-HA+/– / RIP-HA+/– mice compared to BALB/c mice.
机译：尽管周围型糖尿病性神经病的患病率增加，但其某些机制仍是未知的。在我们的研究中，我们使用了双重转基因小鼠（dTg）模型TCR-HA + / – / RIP-HA +/–，该模型发展为胰岛素依赖型糖尿病的暴发类型。我们研究的目的是揭示神经元原代培养物中糖尿病性神经病的分子机制。通过PCR技术确定负责I型糖尿病的病原体发生的转基因的存在。出生4周后，监测血糖，胰岛素水平和体重。神经原代培养物与背根神经节分离。这些神经元的电生理记录通过膜片钳技术，全细胞配置进行。在我们的研究中，我们记录了感觉神经元中动作电位和超极化激活电流的特征。与BALB / c小鼠相比，TCR-HA + / – / RIP-HA +/–小鼠背根神经节的感觉神经元的电生理特征被证明存在显着差异。

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