Evaluation of First-and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver DiseasesEvaluation of 1st and 2nd-Generation Assays in NANBH

SHIGERU TOKESHI; MICHIO SATA; TERUKO HINO; TATSUYA IDE; SEIJI NOGUCHI; HIROSHI SUZUKI; HARUO MURAOKA; HITOSHI NAKANO; KYUICHI TANIKAWA

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Evaluation of First-and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver DiseasesEvaluation of 1st and 2nd-Generation Assays in NANBH

机译：在非A，非B慢性肝病中检测丙型肝炎病毒抗体的第一代和第二代检测方法的评估NANBH中第一代和第二代检测方法的评估

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The positive rates of the second-generation enzyme-linked immunoassay (2nd-generation assay), and two first-generation immunoassays (C100-3 and KCL-163 assay) to test for anti-HCV antibodies in the serum of patients with non-A, non-B chronic liver disease were determined. The clinical usefulness of these assays was also evaluated. The group positive for the 2nd-generation assay alone was compared with that positive for the 2nd-generation and C100-3 assays with respect to the serum GPT levels determined simultaneously with the antibodies. The latter group showed slightly higher GPT levels. These findings suggest that the 2nd-generation assay is useful for the diagnosis of hepatitis C, and that C100-3 and KCL-163 assays are useful indicators of the activity of hepatic disorders.

机译：第二代酶联免疫测定（第二代测定）和两种第一代免疫测定（C100-3和KCL-163测定）的阳性率可检测非乙肝患者血清中的抗HCV抗体确定了A，非B慢性肝病。还评估了这些测定的临床实用性。就与抗体同时测定的血清GPT水平而言，将仅对第二代测定呈阳性的组与对于第二代和C100-3测定呈阳性的组进行比较。后一组显示GPT略高。这些发现表明第二代测定法可用于丙型肝炎的诊断，而C100-3和KCL-163测定法可作为肝功能异常的有用指标。

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《Kurume Medical Journal》 |1993年第1期|共6页
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SHIGERU TOKESHI; MICHIO SATA; TERUKO HINO; TATSUYA IDE; SEIJI NOGUCHI; HIROSHI SUZUKI; HARUO MURAOKA; HITOSHI NAKANO; KYUICHI TANIKAWA;
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1. Analysis of discordant test results among five second-generation assays for anti-hepatitis C virus antibodies also tested by polymerase chain reaction-RNA assay and other laboratory and clinical tests for hepatitis. [J] . I Nakagiri, K Ichihara, K Ohmoto, Journal of Clinical Microbiology . 1993,第11期

机译：在五种第二代抗丙型肝炎病毒抗体检测方法中，不一致的检测结果也通过聚合酶链反应-RNA检测以及其他肝炎实验室和临床检测方法进行了检测。
2. Evolution of hepatitis G virus infection and antibody response to envelope protein in patients with transfusion-associated non-A, non-B hepatitis. [J] . Tanaka E, Kiyosawa K, Shimoda K, Journal of viral hepatitis. . 1998,第3期

机译：输血相关的非甲，非乙型肝炎患者中丙型肝炎病毒感染的演变和对包膜蛋白的抗体反应。
3. Evolution of hepatitis G virus infection and antibody response to envelope protein in patients with transfusion-associated non-A, non-B hepatitis. [J] . Tanaka E, Kiyosawa K, Shimoda K, Journal of viral hepatitis. . 1998,第3期

机译：输血相关的非甲，非乙型肝炎患者中丙型肝炎病毒感染的演变和对包膜蛋白的抗体反应。
4. COMBINED HEPATITIS C VIRUS ANTIGEN AND ANTIBODY ASSAY ON AN AUTOMATED CHEMILUMINESCENT ANALYZER TO SHORTEN SEROCONVERSION WINDOW [C] . DINESH O SHAH, LILY JIANG, KEVIN CHENG, 12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK . 2002

机译：全自动化学发光分析仪结合肝炎病毒C抗原和抗体评估以缩短血清转化率
5. Development and validation of a blocking enzyme-linked immunosorbent assay for the detection of antibodies against porcine reproductive and respiratory syndrome virus. [D] . Ferrin, Neal Harold. 2004

机译：开发和验证用于检测针对猪繁殖与呼吸综合征病毒的抗体的封闭酶联免疫吸附法。
6. Analysis of discordant test results among five second-generation assays for anti-hepatitis C virus antibodies also tested by polymerase chain reaction-RNA assay and other laboratory and clinical tests for hepatitis. [O] . I Nakagiri, K Ichihara, K Ohmoto, 1993

机译：在五种第二代抗丙型肝炎病毒抗体检测方法中不一致的检测结果分析也通过聚合酶链反应-RNA检测以及其他肝炎实验室和临床检测方法进行了检测。
7. Evaluation of First- and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver Diseases. Evaluation of 1st and 2nd-Generation Assays in NANBH. [O] . SHIGERU TOKESHI, MICHIO SATA, TERUKO HINO, 1993

机译：评估用于检测非A，非B慢性肝病中丙型肝炎病毒抗体的第一和第二代测定。纳米第1和第2代测定的评价。
8. Solid-Phase Antibody Capture Hemadsorption Assay for Detection of Hepatitis AVirus Immunoglobulin M Antibodies [R] . Summers, P. L., DuBois, D. R., Cohen, W. H., 1993

机译：用于检测肝炎病毒免疫球蛋白m抗体的固相抗体捕获血细胞吸附试验

Evaluation of First-and Second-Generation Assays for Detection of Antibody to Hepatitis C Virus in non-A, non-B Chronic Liver DiseasesEvaluation of 1st and 2nd-Generation Assays in NANBH

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