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首页> 外文期刊>Open Journal of Animal Sciences >Comparative Expression Profiling of Lactogenic Hormone Receptor and It’s Signaling Molecules of Bovine Mammary Glands during lactation
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Comparative Expression Profiling of Lactogenic Hormone Receptor and It’s Signaling Molecules of Bovine Mammary Glands during lactation

机译：泌乳期泌乳激素激素受体及其信号分子的比较表达谱

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Milk synthesis is known to be modulated by peptide hormones such as prolactin (PRL), growth hormone (GH), and insulin-like growth factor I (IGF-I). Previous studies suggested that PRL and IGF-I acted directly on mammary epithelial cells and were involved in lactation. Meanwhile, GH is thought to be indirectly involved in lactation by stimulating the secretion of IGF-I. It is controversial as growth hormone receptors (GHR) is expressed in the mammary epithelial cells. In order to clarify whether GH acted directly on mammary gland tissue, we investigated the prolactin receptors (PRLR), IGF-I receptors (IGF-IR), and GHR as well as the gene expression levels of the downstream signaling molecule for each receptor in the mammary gland tissue of Holstein cows during different stages of lactation. The results revealed that the mRNA expressions of PRLR and IGF-IR were highest during early lactation, and the mRNA expression of the GHR was highest during mid-lactation. We also found that the expression profiling of the signal transducer and activator of transcription 5 (STAT5) genes was similar to that of the GHR gene. On the other hand, the expression profiling of the PRLR gene was similar to that of the SHP2 gene. These results suggest that GH acts on the mammary glands directly, milk synthesis and secretion are chiefly stimulated in mid-lactation, and the timing of the action is different for PRL and IGF-I.
机译：已知牛奶合成受肽激素如催乳激素（PRL），生长激素（GH）和胰岛素样生长因子I（IGF-1）调节。先前的研究表明PRL和IGF-I直接作用于乳腺上皮细胞并参与泌乳。同时，认为GH通过刺激IGF-I的分泌间接参与泌乳。由于生长激素受体（GHR）在乳腺上皮细胞中表达，因此存在争议。为了阐明GH是否直接作用于乳腺组织，我们研究了催乳素受体（PRLR），IGF-I受体（IGF-IR）和GHR以及下游信号分子在每个受体中的基因表达水平。泌乳不同阶段荷斯坦奶牛的乳腺组织。结果表明，泌乳初期PRLR和IGF-1R的mRNA表达最高，泌乳中期GHR的mRNA表达最高。我们还发现，信号转导子和转录激活子5（STAT5）基因的表达谱与GHR基因的表达谱相似。另一方面，PRLR基因的表达谱与SHP2基因相似。这些结果表明，GH直接作用于乳腺，在泌乳中期主要刺激乳的合成和分泌，并且PRL和IGF-I的作用时间不同。

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