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首页> 外文期刊>Open Journal of Genetics >Microbial community diversity analysis of Panax ginseng rhizosphere and non-rhizosphere soil using randomly amplified polymorphic DNA method
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Microbial community diversity analysis of Panax ginseng rhizosphere and non-rhizosphere soil using randomly amplified polymorphic DNA method

机译：人参根际和非根际土壤微生物群落多样性的随机扩增多态性DNA分析

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摘要

DNA sequence diversities of soil microbial communities in rhizosphere and non-rhizosphere of 1 - 6 years Panax ginseng were evaluated by random amplified polymorphic DNA (RAPD). Total genomic DNA of soil samples were amplified by twenty-four out of two hundred random primers. The products were separated in agarose gel, and 359 and 181 reliable fragments were generated, of which, 324 and 123 were polymorphic. The gene diversity index, Shannon’s information index, and similarity coefficient were calculated, and results showed that, diversity of soil microbial community at DNA level was present. Furthermore, with the increasing of P. ginseng growing at one site, deference on soil microbial community DNA sequence between rhizosphere and non-rhizosphere tend to be more and more significant. In addition, RAPD technique was proved to be an effective tool to assess the diversity of soil microbial communities. From results we deduced that, the ecological function that performed by soil microbes may changed following the change of microbial community in rhizospheric and non-rhizospheric soils, and finally broke the balance of soil nutrition and energy cycle, soil spatial structure, microbial ecology etc.
机译：通过随机扩增多态性DNA（RAPD）评估了人参1-6年根际和非根际土壤微生物群落的DNA序列多样性。用200种随机引物中的24种扩增土壤样品的总基因组DNA。用琼脂糖凝胶分离产物，并产生359和181个可靠的片段，其中324和123是多态的。计算了基因多样性指数，香农信息指数和相似系数，结果表明在DNA水平上存在土壤微生物群落的多样性。此外，随着人参一处生长的增加，对根际和非根际之间土壤微生物群落DNA序列的尊重越来越重要。此外，RAPD技术被证明是评估土壤微生物群落多样性的有效工具。从结果推断，根际土壤和非根际土壤中微生物的生态功能可能会随着微生物群落的变化而发生变化，最终破坏土壤营养与能量循环，土壤空间结构，微生物生态等方面的平衡。

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