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首页> 外文期刊>Open Journal of Biophysics >Effects of Storage Duration and Temperature of Human Plasma and Serum on Red Blood Cell Aggregation and Shape
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Effects of Storage Duration and Temperature of Human Plasma and Serum on Red Blood Cell Aggregation and Shape

机译：血浆和血清的储存时间和温度对红细胞聚集和形态的影响

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摘要

Platelet-free plasma of human blood (sodium citrate and EDTA as an anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at 37°C for 24 hours. RBC aggregation decreased after incubation of plasma and serum at 37°C for 4 hours. The RBC shape was changed at the same time: discocytes transformed to echinocytes. Storage of plasma and serum at 4°C and room temperature did not lead to significant alterations of RBC aggregation. The RBC shape did not change in influence of such plasma and serum. The most considerable decrease of RBC aggregation and change of their shapes were observed in the plasma and serum incubated at 37°C for 24 hours. Dilution of incubated plasma by fresh plasma led to consistent restoration of erythrocyte shape and their aggregation.
机译：人血的无血小板血浆（柠檬酸钠和EDTA作为抗凝剂）和血清分别在4°C，室温（25°C）和37°C下保存24小时。将血浆和血清在37°C孵育4小时后，RBC聚集减少。 RBC形状同时改变：盘状细胞转化为棘突细胞。血浆和血清在4°C和室温下的储存不会导致RBC聚集的显着改变。在这种血浆和血清的影响下，RBC的形状没有改变。在37°C孵育24小时的血浆和血清中，观察到RBC聚集和形状变化的最大幅度降低。用新鲜血浆稀释培养的血浆可导致红细胞形状及其聚集的一致恢复。

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