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首页> 外文期刊>Open access Insect Physiology >Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)
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Transcription profiling of guanine nucleotide binding proteins during developmental regulation and pesticide response in Solenopsis invicta (Hymenoptera: Formicidae)

机译：鸟巢菌发育调节和杀虫剂响应过程中鸟嘌呤核苷酸结合蛋白的转录谱分析（膜翅目：For科）

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Abstract: Guanine nucleotide binding proteins (GNBP), glycoproteins anchored on the cytoplasmic cell membrane, are mediators for many cellular processes. Complete cDNA of the GNBP gene β-subunit (SiGNBP) was cloned and sequenced from Solenopsis invicta Buren (Hymenoptera: Formicidae) worker ants. To understand whether SiGNBP is developmentally regulated in S. invicta, its expression levels in different developmental stages were examined using quantitative real-time polymerase chain reaction. SiGNBP was expressed in each developmental stage, and was especially highly expressed in the late larval and early pupal stages, as well among the dealate females (queens) ~10 days post nuptial flight. Quantitative real-time polymerase chain reaction also showed that mRNA transcription levels of SiGNBP in S. invicta workers were regulated during the duration of the study in response to heat shock, ultraviolet light, and boric acid treatment. These results suggest that the SiGNBP gene may play an important role in the development of S. invicta, and this has potential for use as a target in new insecticides for the control of fire ants.
机译：摘要：鸟嘌呤核苷酸结合蛋白（GNBP）是锚定在细胞质细胞膜上的糖蛋白，是许多细胞过程的介体。从工蚁蚁（膜翅目：蚁科）中克隆了GNBP基因β亚基（SiGNBP）的完整cDNA并进行了测序。为了了解SiGNBP是否在S. invicta中得到发育调控，使用定量实时聚合酶链反应检测了其在不同发育阶段的表达水平。 SiGNBP在每个发育阶段都有表达，在幼虫后期和p早期以及婚后飞行约10天后的雌性雌性（雌性）中特别高表达。实时定量聚合酶链反应还显示，在研究期间，响应热休克，紫外线和硼酸处理，S。invicta工人中SiGNBP的mRNA转录水平受到调节。这些结果表明，SiGNBP基因可能在S. invicta的发育中起重要作用，并且有潜力用作控制火蚁的新型杀虫剂的靶标。

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