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首页> 外文期刊>Oncogene >A 1|[thinsp]|Mb minimal amplicon at 8p11|[ndash]|12 in breast cancer identifies new candidate oncogenes
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A 1|[thinsp]|Mb minimal amplicon at 8p11|[ndash]|12 in breast cancer identifies new candidate oncogenes

机译：乳腺癌中8p11 | nb | 12处的1 | [thinsp] | Mb最小扩增子识别出新的候选癌基因

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摘要

Amplification of 8p11–12 is a well-known alteration in human breast cancers but the driving oncogene has not been identified. We have developed a high-resolution comparative genomic hybridization array covering 8p11–12 and analysed 33 primary breast tumors, 20 primary ovarian tumors and 27 breast cancer cell lines. Expression analysis of the genes in the region was carried out by using real-time quantitative PCR and/or oligo-microarray profiling. In all, 24% (8/33) of the breast tumors, 5% (1/20) of the ovary tumors and 15% (4/27) of the cell lines showed 8p11–12 amplification. We identified a 1Mb segment of common amplification that excludes previously proposed candidate genes. Some of the amplified genes did not show overexpression, whereas for others, overexpression was not specifically attributable to amplification. The genes FLJ14299, C8orf2, BRF2 and RAB11FIP, map within the 8p11–12 minimal amplicon, two have a putative function consistent with an oncogenic role, these four genes showed a strong correlation between amplification and overexpression and are therefore the best candidate driver oncogenes at 8p12.
机译：8p11-12的扩增是人类乳腺癌中的一个众所周知的变化，但尚未确定驱动癌基因。我们已经开发了一种覆盖8p11-12的高分辨率比较基因组杂交阵列，并分析了33种原发性乳腺肿瘤，20种原发性卵巢肿瘤和27种乳腺癌细胞系。通过使用实时定量PCR和/或寡微阵列分析对区域中的基因进行表达分析。总共有24％（8/33）的乳腺肿瘤，5％（1/20）的卵巢肿瘤和15％（4/27）的细胞系显示8p11–12扩增。我们确定了一个普通扩增的1Mb片段，该片段不包括先前提出的候选基因。一些扩增的基因没有显示出过表达，而对于另一些，过度表达并不是特异性地归因于扩增。基因FLJ14299，C8orf2，BRF2和RAB11FIP在8p11–12最小扩增子中作图，两个具有推定的功能与致癌作用相一致，这四个基因显示出扩增和过表达之间的强相关性，因此是在8p12。

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