• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Oncogene >Protein kinase CK2 regulates CDC25B phosphatase activity
【24h】

Protein kinase CK2 regulates CDC25B phosphatase activity

机译：蛋白激酶CK2调节CDC25B磷酸酶活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human dual-specificity phosphatases CDC25 (A, B and C) play an important role in the control of cell cycle progression by activating the cyclin-dependent kinases (CDKs). Regulation of these phosphatases during the cell cycle involves post-translational modifications such as phosphorylation and protein–protein interactions. Given the suspected involvement of the protein kinase CK2 at the G2/M transition, we have investigated its effects on the CDC25B phosphatase. We show that in vitro CK2 phosphorylates CDC25B, but not CDC25C. Mass spectrometry analysis demonstrates that at least two serine residues, Ser-186 and Ser-187, are phosphorylated in vivo. We also report that CDC25B interacts with CK2, and this interaction, mediated by the CK2 regulatory subunit, involves domains that are located within the first 55 amino acids of CK2 and between amino acids 122 and 200 on CDC25B. This association was confirmed in vivo, in Sf9 insect cells and in U2OS human cells expressing an HA epitope-tagged CDC25B. Finally, we demonstrate that phosphorylation of CDC25B by protein kinase CK2 increases the catalytic activity of the phosphatase in vitro as well as in vivo. We discuss the possibility that CDC25B phosphorylation by CK2 could play a role in the regulation of the activity of CDC25B as a starter of mitosis.
机译：人类双特异性磷酸酶CDC25（A，B和C）通过激活细胞周期蛋白依赖性激酶（CDK）在控制细胞周期进程中发挥重要作用。这些磷酸酶在细胞周期中的调节涉及翻译后修饰，例如磷酸化和蛋白质-蛋白质相互作用。考虑到蛋白激酶CK2可能参与了G2 / M过渡，我们研究了其对CDC25B磷酸酶的作用。我们显示体外CK2磷酸化CDC25B，但不是CDC25C。质谱分析表明，至少两个丝氨酸残基Ser-186和Ser-187在体内被磷酸化。我们还报告说CDC25B与CK2相互作用，并且这种相互作用由CK2调节亚基介导，涉及位于CK2的前55个氨基酸内以及CDC25B的第122和200位氨基酸之间的域。在体内，在表达HA表位标记的CDC25B的Sf9昆虫细胞和U2OS人类细胞中证实了这种联系。最后，我们证明了蛋白激酶CK2对CDC25B的磷酸化作用增加了体外和体内磷酸酶的催化​​活性。我们讨论了由CK2引起的CDC25B磷酸化可能在CDC25B作为有丝分裂起始剂的活性调节中发挥作用的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号