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首页> 外文期刊>Oncogene >Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains
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Carom: a novel membrane-associated guanylate kinase-interacting protein with two SH3 domains

机译：Carom：具有两个SH3域的新型膜相关鸟苷酸激酶相互作用蛋白

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摘要

MAGI-1 and CASK are membrane-associated guanylate kinases of epithelial junctions. MAGI-1 is localized at tight junctions in polarized epithelial cells, whereas CASK is localized along the lateral membranes. We obtained the KIAA0769 gene product through the yeast two-hybrid screening using MAGI-1 as a bait and named it Carom. Carom has a coiled-coil domain in the middle region, and two src homology 3 domains and a PSD-95/Dlg-A/ZO-1 (PDZ)-binding motif in the C-terminal region. Carom binds to the fifth PDZ domain of MAGI-1 and the calmodulin kinase domain of CASK in vitro. MAGI-1 and CASK bind to the distinct sequences in the C-terminal region of Carom, but still compete with each other for Carom binding. The study using a stable transformant of Madine Darby canine kidney (MDCK) cells expressing GFP-Carom revealed that Carom was partially overlapped by MAGI-1 in MDCK cells, which have not yet established mature cell junctions, but became separated from MAGI-1 and colocalized with CASK in polarized cells. Carom was highly resistant to Triton X-100 extractions and recruited CASK to the Triton X-100-insoluble structures. Carom is a binding partner of CASK, which interacts with CASK in polarized epithelial cells and may link it to the cytoskeleton.
机译：MAGI-1和CASK是上皮连接的膜相关鸟苷酸激酶。 MAGI-1定位在极化上皮细胞的紧密连接处，而CASK则沿着侧膜定位。我们使用MAGI-1作为诱饵，通过酵母双杂交筛选获得了KIAA0769基因产物，并将其命名为Carom。 Carom在中间区域具有卷曲螺旋结构域，在C末端区域具有两个src同源3结构域和PSD-95 / Dlg-A / ZO-1（PDZ）结合基序。 Carom在体外与MAGI-1的第五个PDZ域和CASK的钙调蛋白激酶域结合。 MAGI-1和CASK结合到Carom的C末端区域中的不同序列，但仍彼此竞争Carom的结合。使用表达GFP-Carom的Madine Darby犬肾（MDCK）细胞的稳定转化体进行的研究显示，在尚未建立成熟细胞连接的MDCK细胞中，MAGI-1与Carom部分重叠，但与MAGI-1和与CASK在极化细胞中共定位。 Carom对Triton X-100的提取物具有很高的抵抗力，并将CASK募集到Triton X-100不溶的结构中。 Carom是CASK的结合伴侣，它与极化上皮细胞中的CASK相互作用并将其链接到细胞骨架。

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