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首页> 外文期刊>Sao Paulo Medical Journal >Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis
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Enzyme-linked immunosorbent assay (ELISA) immunoassaying versus microscopy: advantages and drawbacks for diagnosing giardiasis

机译：酶联免疫吸附测定（ELISA）免疫测定与显微镜检查：诊断贾第鞭毛虫病的优缺点

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CONTEXT AND OBJECTIVE: Giardiasis is common in Brazil. For laboratory diagnosis, the method most utilized is microscopic examination of fecal samples, but the immunoenzymatic method is also available. The aim of this work was to verify the advantages and drawbacks of immunoassaying versus microscopy for diagnosing Giardia lamblia, when a single fecal sample is analyzed. DESIGN AND SETTING: Prospective, double-blind study at the parasitology laboratory of Faculdade de Medicina da Funda??o ABC. METHODS: Samples were prepared according to the traditional sedimentation (Hoffman, Pons and Janer) and Faust methods. Results were deemed positive when Giardia lamblia was found by one or both methods. The Prospect ELISA kit was used for detecting Giardia lamblia-specific antigen, in accordance with the manufacturer's instructions. Results were expressed on a visual scale as negative or positive (+, ++, +++ or ++++). RESULTS: The ELISA test was positive even when a significant proportion of corresponding samples examined by microscopy were negative. This trend was statistically significant (p < 0.001). The overall concordance of results between the ELISA test and microscopic examination of single samples was only moderate (0.50 by kappa test). CONCLUSION: The ELISA test is useful when just searching for Giardia lamblia, because of its high sensitivity. For daily practice, we recommend microscopy, which is much cheaper and can also detect other parasites. The low positivity of single samples in this method can be overcome by using three samples, as recommended by most authors.
机译：语境和目的：贾第鞭毛虫病在巴西很常见。对于实验室诊断，最常用的方法是对粪便样本进行显微镜检查，但也可以使用免疫酶法。这项工作的目的是在分析单个粪便样本时，验证免疫分析与显微镜检查在诊断贾第鞭毛虫中的优缺点。设计与环境：在Faculdade de Medicina da Funda ?? o ABC的寄生虫学实验室进行前瞻性，双盲研究。方法：根据传统的沉降（霍夫曼，庞斯和詹纳）和浮士德方法制备样品。当通过一种或两种方法发现贾第鞭毛虫时，结果被认为是阳性的。根据制造商的说明，将Prospect ELISA试剂盒用于检测贾第鞭毛虫特异性抗原。结果在视觉上表示为阴性或阳性（+，++，+++或++++）。结果：即使显微镜检查的相当多的相应样品为阴性，ELISA测试仍为阳性。该趋势具有统计学意义（p <0.001）。 ELISA试验与单个样品的显微镜检查结果的总体一致性仅为中等水平（kappa试验为0.50）。结论：ELISA检测法在寻找兰氏贾第鞭毛虫时非常有用，因为它具有很高的灵敏度。对于日常实践，我们建议您使用显微镜，它便宜得多，并且还可以检测其他寄生虫。如大多数作者所建议的那样，可以通过使用三个样本来克服此方法中单个样本的阳性率低的问题。

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