...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Sao Paulo Medical Journal >Red cell aspartate aminotransferase saturation with oral pyridoxine intake
【24h】

Red cell aspartate aminotransferase saturation with oral pyridoxine intake

机译：口服吡ido醇摄入会导致红细胞天冬氨酸转氨酶饱和

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

CONTEXT AND OBJECTIVE: The coenzyme of aspartate aminotransferase is pyridoxal phosphate, generated from fresh vegetables containing pyridoxine. Vitamin B6-responsive sideroblastic anemia, myelofibrosis and Peyronie’s syndrome respond to high pyridoxine doses. The objective was to investigate the oral pyridoxine oral dose that would lead to maximized pyridoxal phosphate saturation of red cell aspartate aminotransferase. DESIGN AND SETTING: Controlled trial, in Hematology Division of Instituto Adolfo Lutz. METHODS: Red cell aspartate aminotransferase activity was assayed (before and after) in normal volunteers who were given oral pyridoxine for 15-18 days (30 mg, 100 mg and 200 mg daily). In vitro study of blood from seven normal volunteers was also performed, with before and after assaying of aspartate aminotransferase activity. RESULTS: The in vivo study showed increasing aspartate aminotransferase saturation with increasing pyridoxine doses. 83% saturation was reached with 30 mg daily, 88% with 100 mg, and 93% with 200 mg after 20 days of oral supplementation. The in vitro study did not reach 100% saturation. CONCLUSIONS: Neither in vivo nor in vitro study demonstrated thorough aspartate aminotransferase saturation with its coenzyme pyridoxal phosphate in red cells, from increasing pyridoxine supplementation. However, the 200-mg dose could be employed safely in vitamin B6-responsive sideroblastic anemia, myelofibrosis and Peyronie’s syndrome treatment. Although maximum saturation in circulating red cells is not achieved, erythroblasts and other nucleated and cytoplasmic organelles containing cells certainly will reach thorough saturation, which possibly explains the results obtained in these diseases.
机译：上下文和目的：天冬氨酸转氨酶的辅酶是磷酸吡ido醛，由含有吡ido醇的新鲜蔬菜产生。维生素B6引起的铁粒幼细胞性贫血，骨髓纤维化和佩罗尼氏综合症对高吡high醇剂量有反应。目的是研究口服吡ido醇口服剂量，该剂量将导致红细胞天冬氨酸氨基转移酶的吡ido醛磷酸盐饱和度最大化。设计与设置：Adolfo Lutz研究所血液科的对照试验。方法：在接受口服吡ido醇治疗15-18天（每天30 mg，100 mg和200 mg）的正常志愿者中（之前和之后）测定红细胞天冬氨酸转氨酶的活性。在测定天冬氨酸转氨酶活性之前和之后，还进行了来自七个正常志愿者的血液的体外研究。结果：体内研究显示，随着吡ido醇剂量的增加，天冬氨酸转氨酶饱和度增加。口服补充20天后，每天30 mg，83％100 mg和93％200 mg达到83％的饱和度。体外研究未达到100％饱和度。结论：体内和体外研究均未证明由于补充吡ido醇而使红细胞中的天冬氨酸转氨酶及其辅酶吡ido醛磷酸盐充分饱和。但是，可以将200 mg的剂量安全地用于对维生素B6有反应的铁粒幼细胞性贫血，骨髓纤维化和Peyronie综合征的治疗。尽管没有达到循环红细胞的最大饱和度，但含有细胞的成红细胞和其他有核细胞质和细胞质细胞器肯定会达到完全饱和，这可能解释了在这些疾病中获得的结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号