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首页> 外文期刊>Sao Paulo Medical Journal >Human herpesvirus 8 (HHV-8) detected by nested polymerase chain reaction (PCR) in HIV patients with or without Kaposi's sarcoma. An analytic cross-sectional study
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Human herpesvirus 8 (HHV-8) detected by nested polymerase chain reaction (PCR) in HIV patients with or without Kaposi's sarcoma. An analytic cross-sectional study

机译：通过巢式聚合酶链反应（PCR）在有或没有卡波西肉瘤的HIV患者中检测到人类疱疹病毒8（HHV-8）。解析横断面研究

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CONTEXT AND OBJECTIVE: Kaposi's sarcoma (KS) is a common neoplastic disease in AIDS patients. The aim of this study was to evaluate the frequency of human herpesvirus 8 (HHV-8) infection in human immunodeficiency virus (HIV)-infected patients, with or without KS manifestations and correlate HHV-8 detection with KS staging. DESIGN AND SETTING: Analytic cross-sectional study conducted in a public tertiary-level university hospital in Ribeirão Preto, São Paulo, Brazil. METHODS: Antibodies against HHV-8 lytic-phase antigens were detected by means of the immunofluorescence assay. HHV-8 DNA was detected in the patient samples through a nested polymerase chain reaction (nested PCR) that amplified a region of open reading frame (ORF)-26 of HHV-8. RESULTS: Anti-HHV-8 antibodies were detected in 30% of non-KS patients and 100% of patients with KS. Furthermore, the HHV-8 DNA detection rates observed in HIV-positive patients with KS were 42.8% in serum, 95.4% in blood samples and 100% in skin biopsies; and in patients without KS, the detection rate was 4% in serum. Out of the 16 serum samples from patients with KS-AIDS who were classified as stage II, two were positive (12.5%); and out of the 33 samples from patients in stage IV, 19 (57.6%) were positive. CONCLUSION: We observed an association between HHV-8 detection and disease staging, which was higher in the serum of patients in stage IV. This suggests that detection of HHV-8 DNA in serum could be very useful for clinical assessment of patients with KS and for monitoring disease progression.
机译：背景与目的：卡波济肉瘤（KS）是艾滋病患者中常见的肿瘤性疾病。这项研究的目的是评估在人类免疫缺陷病毒（HIV）感染的患者中有或没有KS表现的人类疱疹病毒8（HHV-8）感染的频率，并将HHV-8检测与KS分期相关联。设计与环境：分析性横断面研究是在巴西圣保罗的里贝朗·普雷图的一家三级公立大学医院进行的。方法：通过免疫荧光法检测针对HHV-8裂解期抗原的抗体。通过巢式聚合酶链反应（巢式PCR）在患者样本中检测到HHV-8 DNA，该反应扩增了HHV-8的开放阅读框（ORF）-26区域。结果：30％的非KS患者和100％的KS患者检测到抗HHV-8抗体。此外，在HIV阳性KS患者中观察到的HHV-8 DNA检出率为血清的42.8％，血液样本的95.4％和皮肤活检的100％。在没有KS的患者中，血清检出率为4％。在被归为II期的KS-AIDS患者的16份血清样本中，有2份呈阳性（12.5％）。在IV期患者的33份样本中，有19份（57.6％）为阳性。结论：我们观察到HHV-8检测与疾病分期之间的关联，在IV期患者的血清中更高。这表明在血清中检测HHV-8 DNA对KS患者的临床评估和监测疾病进展可能非常有用。

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