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首页> 外文期刊>Saudi Journal of Biological Sciences >Phylogenetic analyses of peanut resistance gene candidates and screening of different genotypes for polymorphic markers
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Phylogenetic analyses of peanut resistance gene candidates and screening of different genotypes for polymorphic markers

机译：花生抗性候选基因的系统发育分析及多态性标记不同基因型的筛选

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The nucleotide-binding-site-leucine-rich-repeat (NBS-LRR)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. Here, we describe a collection of peanut NBS-LRR resistance gene candidates (RGCs) isolated from peanut (Arachis) species by mining Gene Bank data base. NBS-LRR sequences assembled into TIR-NBS-LRR (75.4%) and non-TIR-NBS-LRR (24.6%) subfamilies. Total of 20 distinct clades were identified and showed a high level of sequence divergence within TIR-NBS and non-TIR-NBS subfamilies. Thirty-four primer pairs were designed from these RGC sequences and used for screening different genotypes belonging to wild and cultivated peanuts. Therefore, peanut RGC identified in this study will provide useful tools for developing DNA markers and cloning the genes for resistance to different pathogens in peanut.
机译：编码核苷酸结合位点的富含亮氨酸重复序列（NBS-LRR）的基因家族吸引了许多研究兴趣，因为迄今为止已克隆的植物抗病基因中约有75％来自该基因家族。在这里，我们描述了通过挖掘基因库数据库从花生（花生）物种中分离出来的花生NBS-LRR抗性基因候选物（RGC）的集合。 NBS-LRR序列组装为TIR-NBS-LRR（75.4％）和非TIR-NBS-LRR（24.6％）亚家族。总共鉴定出20个不同的进化枝，并在TIR-NBS和非TIR-NBS亚家族中显示出高水平的序列差异。从这些RGC序列设计了34对引物对，并用于筛选属于野生和栽培花生的不同基因型。因此，本研究中鉴定的花生RGC将为开发DNA标记和克隆抗花生中不同病原体的基因提供有用的工具。

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