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首页> 外文期刊>Saudi Journal of Biological Sciences >Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes
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Computational identification and characterization of miRNAs and their target genes from five cyprinidae fishes

机译：五种鲤科鱼类miRNA及其靶基因的计算鉴定与表征

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MicroRNAs (miRNAs) are a kind of small single-strand RNA molecules with lengths of 18-25nt, which do not encode any proteins. They play an essential role in gene expression regulation by binding to their target genes, leading to translational repression or transcript degradation. In this study, 23 miRNAs were predicted from five cyprinidae fishes by using a bioinformatics-based gene search based on blasting ESTs and GSS in NCBI, of which 21 miRNA genes have not been previously reported. To prove their validity, five of the computationally predicted miRNAs were verified by RTPCR, their transcripts were successfully detected, and, 46 potential target genes for these miRNAs were predicted, most target genes encode transcription factors, they are involved in signal transduction, metabolism and development processes.
机译：微小RNA（miRNA）是一种小的单链RNA分子，长度为18-25nt，不编码任何蛋白质。通过与靶基因结合，它们在基因表达调控中起着至关重要的作用，导致翻译抑制或转录产物降解。在这项研究中，通过使用基于生物信息学的基因搜索基于NCBI中的EST和GSS爆破，从五种鲤科鱼类中预测了23种miRNA，其中先前未报道过21种miRNA基因。为了证明其有效性，通过RTPCR验证了5个计算预测的miRNA，成功检测了其转录本，并预测了这些miRNA的46个潜在靶基因，大多数靶基因编码转录因子，它们参与信号转导，代谢和代谢。开发过程。

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