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首页> 外文期刊>Science China Life Sciences >Comparative proteome analysis of alkaliphilic Bacillus sp. N16-5 grown on different carbon sources
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Comparative proteome analysis of alkaliphilic Bacillus sp. N16-5 grown on different carbon sources

机译：碱性芽孢杆菌的比较蛋白质组学分析。 N16-5在不同碳源上生长

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摘要

To determine the impact of carbohydrates on the metabolic pathway in alkaliphiles, proteomes were obtained from cultures containing different carbohydrates and were resolved on two-dimensional gel electrophoresis (2-DE). The proteomes were compared to determine differentially expressed proteins. A novel alkaliphilic bacterium (alkaliphilic Bacillus sp. N16-5 isolated from Wudunur Soda Lake, China) was isolated in media with five different carbon sources (glucose, mannose, galactose, arabinose, and xylose). Comparative proteome analysis identified 61 differentially expressed proteins, which were mainly involved in carbohydrate metabolism, amino acid transport, and metabolism, as well as energy production and conversion. The comparison was based on the draft genome sequence of strain N16-5. The abundance of enzymes involved in central metabolism was significantly changed when exposed to various carbohydrates. Notably, catabolite control protein A (CcpA) was up-regulated under all carbon sources compared with glucose. In addition, pentose exhibited a stronger effect than hexose in CcpA-mediated carbon catabolite repression. These results provided a fundamental understanding of carbohydrate metabolism in alkaliphiles.
机译：为了确定碳水化合物对亲碱代谢途径的影响，从含有不同碳水化合物的培养物中获得蛋白质组，并在二维凝胶电泳（2-DE）上进行解析。比较蛋白质组以确定差异表达的蛋白质。在具有五种不同碳源（葡萄糖，甘露糖，半乳糖，阿拉伯糖和木糖）的培养基中分离了一种新型的嗜碱细菌（从中国武当湖苏打水分离的嗜碱芽孢杆菌N16-5）。比较蛋白质组分析确定了61种差异表达的蛋白质，这些蛋白质主要参与碳水化合物的代谢，氨基酸转运和代谢以及能量的产生和转化。比较是基于菌株N16-5的基因组序列草图。当暴露于各种碳水化合物时，参与中枢代谢的酶的丰度发生了显着变化。值得注意的是，与葡萄糖相比，在所有碳源下分解代谢物对照蛋白A（CcpA）均被上调。此外，戊糖在CcpA介导的碳分解代谢物阻抑中显示出比己糖更强的作用。这些结果提供了对亲碱中碳水化合物代谢的基本理解。

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