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首页> 外文期刊>Skeletal Muscle >Intracellular Ca 2+ -handling differs markedly between intact human muscle fibers and myotubes
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Intracellular Ca 2+ -handling differs markedly between intact human muscle fibers and myotubes

机译：完整的人类肌肉纤维和肌管之间的细胞内Ca 2 + 处理明显不同

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Background In skeletal muscle, intracellular Ca 2+ is an important regulator of contraction as well as gene expression and metabolic processes. Because of the difficulties to obtain intact human muscle fibers, human myotubes have been extensively employed for studies of Ca 2+ -dependent processes in human adult muscle. Despite this, it is unknown whether the Ca 2+ -handling properties of myotubes adequately represent those of adult muscle fibers. Methods To enable a comparison of the Ca 2+ -handling properties of human muscle fibers and myotubes, we developed a model of dissected intact single muscle fibers obtained from human intercostal muscle biopsies. The intracellular Ca 2+ -handling of human muscle fibers was compared with that of myotubes generated by the differentiation of primary human myoblasts obtained from vastus lateralis muscle biopsies. Results The intact single muscle fibers all demonstrated strictly regulated cytosolic free [Ca 2+ ] ([Ca 2+ ] i ) transients and force production upon electrical stimulation. In contrast, despite a more mature Ca 2+ -handling in myotubes than in myoblasts, myotubes lacked fundamental aspects of adult Ca 2+ -handling and did not contract. These functional differences were explained by discrepancies in the quantity and localization of Ca 2+ -handling proteins, as well as ultrastructural differences between muscle fibers and myotubes. Conclusions Intact single muscle fibers that display strictly regulated [Ca 2+ ] i transients and force production upon electrical stimulation can be obtained from human intercostal muscle biopsies. In contrast, human myotubes lack important aspects of adult Ca 2+ -handling and are thus an inappropriate model for human adult muscle when studying Ca 2+ -dependent processes, such as gene expression and metabolic processes.
机译：背景技术在骨骼肌中，细胞内Ca 2+是收缩以及基因表达和代谢过程的重要调节剂。由于难以获得完整的人类肌肉纤维，人类肌管已被广泛用于研究人类成年肌肉中Ca 2+依赖性过程。尽管如此，尚不清楚肌管的Ca 2+处理特性是否能充分代表成年肌纤维的特性。方法为了能够比较人肌肉纤维和肌管的Ca 2+处理特性，我们建立了从人肋间肌肉活检获得的解剖完整单条肌纤维的模型。将人肌肉纤维的细胞内Ca 2+处理与通过从股外侧肌活检获得的原代人成肌细胞的分化产生的肌管进行比较。结果完整的单条肌纤维均显示出严格调节的无胞质[Ca 2+]（[Ca 2+] i）瞬变，并在电刺激下产生力。相反，尽管肌管中的Ca 2+处理要比成肌细胞中成熟，但肌管缺乏成年Ca 2+处理的基本方面并且没有收缩。这些功能差异是由Ca 2+处理蛋白的数量和位置差异以及肌肉纤维和肌管之间的超微结构差异所解释的。结论可以从人肋间肌肉活组织检查中获得完整的单条肌纤维，其显示出严格调节的[Ca 2+] i瞬变并在电刺激时产生力。相比之下，人类肌管缺乏成人Ca 2+处理的重要方面，因此在研究Ca 2+依赖性过程（如基因表达和代谢过程）时，对于成年肌肉是不合适的模型。

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