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Isolation and screening of rhizobia for auxin biosynthesis and growth promotion of mung bean (Vigna radiata L.) seedlings under axenic conditions

机译:生根条件下绿根菌生长素的分离与筛选及其对绿豆幼苗生长的促进作用

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A series of screening experiments to evaluate the effectiveness of rhizobia for producing auxins and improve growth and nodulation of mungbean (Vigna radiata L.) were carried out under axenic conditions. Forty four iolatess of rhizobia were isolated using standard procedures. Auxin biosynthesis by these rhizobial isolates was determined in the absence and presence of L-Trp, a physiological precursor of auxins. Rhizobial isolates varied widely in auxins biosynthesis capabilities. On the basis of auxins biosynthesis, a pouch experiment was conducted for screening thirty four efficient isolates of rhizobia for the growth promotion of mung bean. Results of pouch study showed that inoculation with selected rhizobial isolates increased the root /shoot length, fresh, and dry shoot weight of mung bean up to 33, 59, 71, 148, 107 and 188%, respectively, over untreated control. Further studies are needed under glasshouse and field conditions for confirmation of these results. purp.
机译:在无菌条件下,进行了一系列筛选实验,以评估根瘤菌生产植物生长素的效果,并改善绿豆(Vigna radiata L.)的生长和结瘤。使用标准程序分离出四十四根根瘤菌。在不存在和存在生长素的生理学前体L-Trp的情况下,测定了这些根瘤菌分离物的生长素生物合成。根瘤菌分离物的生长素生物合成能力差异很大。在植物生长素的生物合成基础上,进行了袋装实验,筛选出34种有效的根瘤菌菌株,以促进绿豆的生长。袋装研究的结果表明,与未处理的对照相比,接种所选的根瘤菌分离株可使绿豆的根/茎长,新鲜和干梢重量分别增加至33、59、71、148、107和188%。为了证实这些结果,需要在温室和田间条件下进行进一步研究。 p

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