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首页> 外文期刊>Spanish Journal of Agricultural Research >Identification and characterization of fungi associated with esca in vineyards of the Comunidad Valenciana (Spain)
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Identification and characterization of fungi associated with esca in vineyards of the Comunidad Valenciana (Spain)

机译：西班牙巴伦西亚纳葡萄园的与esca相关的真菌的鉴定和表征

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摘要

Grapevines sampled in the Comunidad Valenciana in Spain were examined for esca symptoms, and the different vine parts (trunk, cane and young vine) were surveyed for the presence of esca-related fungi. The fungal species most frequently identified were the mitosporic fungi Diplodia mutila (Dmu), Phaeoacremonium aleophilum (Pal), Phaeomoniella chlamydospora (Pch) and Phomopsis viticola (Pvi) and the basidiomycetes Fomitiporia mediterranea (Fm) and Stereum hirsutum (Shi). PCR and sequencing using the universal pair primers ITS1-ITS4 confirmed the identification, these techniques proved very useful, particularly in cases where fruiting was not evident and/or pure cultures did not display enough diagnostic traits. Dmu and both basidiomycetes were the most frequent fungi associated with esca in this region. Dmu was the only fungal species found in all vine parts studied, being significantly more present in canes (82%) than in trunks (58%) or shoots (28%). Moreover, both basidiomycetes (50% Fm and 56% Shi) and Pch (32%) were most frequently found in trunks.
机译：检查了在西班牙Comunidad Valenciana取样的葡萄的埃斯卡症状，并调查了不同藤部分（树干，甘蔗和幼藤）中是否存在与埃斯卡有关的真菌。最常被鉴定的真菌种类是线粒体真菌双翅目Diplodia mutila（Dmu），嗜酸乳杆菌（Phaeoccremonium aleophilum）（Pal），衣藻（Phaeomoniella chlamydospora（Pch））和小生单胞菌（Phomopsis viticola）（Pvi）以及担子菌（Fidi）mediterranea（Fm）和Stereum um。使用通用对引物ITS1-ITS4的PCR和测序证实了鉴定，这些技术被证明非常有用，尤其是在结果不明显和/或纯培养物未显示足够的诊断性状的情况下。 Dmu和两种担子菌都是该地区与esca相关的最常见真菌。 Dmu是在所有研究的葡萄藤部分中发现的唯一真菌种类，在藤茎（82％）中的存在明显多于树干（58％）或枝条（28％）。此外，在树干中最常发现担子菌（50％Fm和56％Shi）和Pch（32％）。

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