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首页> 外文期刊>Spanish Journal of Agricultural Research >Genetic variability assessment in ‘Muscat’ grapevines including ‘Muscat of Alexandria’ clones from selection programs
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Genetic variability assessment in ‘Muscat’ grapevines including ‘Muscat of Alexandria’ clones from selection programs

机译：选择方案中“麝香猫”葡萄品种（包括“亚历山大麝香猫”克隆）的遗传变异性评估

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Genetic variability is needed to face environmental changes and pathogen constrains. In addition, the search for intravarietal variability contributes to the avoidance of genetic erosion, preserving clones that are adapted to particular conditions. Variability is also important to diversify grapevine-derived products. In this work, we have analyzed the genetic variability of ‘Muscat germplasm’ including samples from neglected vineyards from Alicante and Valencia provinces, accessions of the germplasm collections of ‘Colección de Vides de El Encín’ (Alcalá de Henares, Madrid) and ‘La Casa de las Vides’ (Agullent, Valencia), accessions supplied by nurseries of Valencia province, and ‘Muscat of Alexandria’ clones selected using differential ampelographic characteristics in selection programs (La Marina, Alicante). Fifteen microsatellites (SSRs) were used to study intervarietal variability. The SSR fingerprinting allowed the identification of some accessions, variants, and synonymies. Amplified Fragment Length Polymorphisms (AFLPs) markers and Microsatellite-AFLPs were used to determine the variability attended in ‘Muscat of Alexandria’ accessions. A CAPs (Cleaved Amplified Polymorphic Sequences) marker, recently developed for the discrimination of ‘Muscat’ flavor genotypes using the SNP1822 GT, was assessed and showed that all the analyzed accessions were ‘Muscat’ flavored. The variation found among the analyzed germplasm is very interesting because variants within ‘Muscat of Alexandria’, ‘Muscat Italia’, and ‘Muscat d’Istambul’ have been identified. In addition, intravarietal genetic variation was found among the analyzed accessions in ‘Muscat of Alexandria’ from selection programs.
机译：面对环境变化和病原体约束，需要遗传变异。另外，寻找品种内变异性有助于避免遗传侵蚀，保留适合于特定条件的克隆。可变性对于葡萄源产品的多样化也很重要。在这项工作中，我们分析了“马斯喀特种质”的遗传变异性，包括来自阿利坎特和巴伦西亚省被忽视的葡萄园的样品，“科尔科西翁·德·维德斯·德恩恩斯·恩”（马德里阿尔卡拉·埃纳雷斯，马德里）和“拉Casa de las Vides（阿瓦伦特，巴伦西亚），瓦伦西亚省苗圃提供的种质，以及在选择程序中使用差异描写特征选择的“亚历山大麝香”克隆（La Marina，阿利坎特）。使用15个微卫星（SSR）研究品种间的变异性。 SSR指纹技术可以识别一些登录名，变体和同义词。使用扩增片段长度多态性（AFLP）标记和微卫星AFLP来确定“亚历山大麝香猫”种质的变异性。最近评估了一种使用SNP1822 G> T来区分“ Muscat”风味基因型的CAPs（切分扩增多态序列）标记，结果表明所有分析的登录品均为“ Muscat”风味。在被分析的种质中发现的变异非常有趣，因为已识别出“亚历山大大帝”，“意大利大帝”和“伊斯坦布尔大帝”中的变异。此外，在选择计划的“亚历山大麝香猫”的分析种质中发现了种内遗传变异。

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