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首页> 外文期刊>Spanish Journal of Agricultural Research >Identification, pathogenicity and distribution of the causal agents of dieback in avocado orchards in Spain
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Identification, pathogenicity and distribution of the causal agents of dieback in avocado orchards in Spain

机译:西班牙鳄梨果园中回死病原菌的鉴定,致病性和分布

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An increased incidence of dieback from branches in several avocado orchards in southern Spain was observed in 2014. Surveys were conducted from May to October 2014, sampling the affected branches to isolate the causal agents. A total of 68 fungal isolates, recovered from ten avocado orchards, were identified, by morphological characterisation and DNA sequencing, as belonging to the genera: Neofusicoccum parvum (50%), Colletotrichum gloeosporioides (17.6%), Neofusicoccum luteum (16.2%), Neofusicoccum australe (13.2%), Neofusicoccum mediterraneum (1.5%) and Lasiodiplodia theobromae (1.5%). A decreasing level of virulence in artificial inoculations on avocado plants was observed in N. parvum, N. luteum, N. mediterraneum, N. australe, C. gloeosporioides and L. theobromae , there were significant differences among N. parvum and the rest of species of this genus, and significant differences were only observed between N. luteum and C. gloeosporioides . The geographical distribution of N. parvum and N. Luteum covers different areas, while C. gloeosporioides and N. australe are located only in the areas around Benamocarra and Vélez-Málaga (southern Spain), while N. mediterraneum and L. theobromae appear only occasionally. This is the first study of avocado branch cankers in Spain which identifies the causal agents and establishes their pathogenicity groups, with N. parvum as the most important causal agent of avocado dieback in this area.
机译:2014年,西班牙南部几个鳄梨果园的分支机构出现枯死的情况有所增加。2014年5月至2014年10月进行了调查,对受影响的分支机构进行了抽样调查,以找出病因。通过形态学表征和DNA测序鉴定出从十个鳄梨果园中回收的总共68个真菌分离株,属于以下属:新镰刀菌(50%),炭疽菌(17.6%),黄褐藻(16.2%),澳洲新镰刀菌(13.2%),地中海新镰刀菌(1.5%)和the叶石楠(1.5%)。在鳄梨,小黄猪,中型猪,N.australe,C.gloeosporioides和L. theobromae中,在鳄梨植物上人工接种的毒力有所降低。该属的种,仅在黄花猪笼草和C. gloeosporioides之间观察到显着差异。细小猪笼草和黄皮猪笼草的地理分布覆盖不同的区域,而球孢梭菌和奥古斯塔猪笼草仅位于贝纳莫卡拉和韦莱斯-马拉加(西班牙南部)附近,而地中海猪笼草和L. theobromae仅出现偶尔。这是西班牙对鳄梨分支溃疡病的首次研究,该研究确定了病因,并确定了它们的致病性群体,小叶猪笼草是该地区鳄梨枯死的最重要病因。

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