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首页> 外文期刊>Spanish Journal of Agricultural Research >Application of near-infrared reflectance spectroscopy for the estimation of protein and L-canavanine contents in seeds of one-flowered vetch (Vicia articulata Hornem.)
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Application of near-infrared reflectance spectroscopy for the estimation of protein and L-canavanine contents in seeds of one-flowered vetch (Vicia articulata Hornem.)

机译:近红外反射光谱法在估计一花紫v(Vicia articulata Hornem。)种子中蛋白质和L-鸟氨酸含量中的应用

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In this work it has been evaluated the effectiveness of near-infrared reflectance spectroscopy (NIRS) for the estimation of two constituents affecting the nutritional quality of the seeds of one-flowered vetch (Vicia articulata Hornem.): protein and L-canavanine, a toxic non-protein amino acid. The NIRS calibrations showed good statistics with coefficients of multiple determination (R2) of 0.97 for total protein and 0.95 for L-canavanine. The developed equations were further used to estimate the contents of protein and L-canavanine of a set of unknown samples (external validation). The equation for total protein was able to predict with an accuracy similar to that of the reference method showing a correlation (r2) between the reference and predicted values of 0.95. In the case of L-canavanine, r2 was only 0.72 and the equation was only effective to discriminate samples into groups of low, medium and high contents.
机译:在这项工作中,已经评估了近红外反射光谱法(NIRS)评估影响一朵紫v菜(Vicia articulata Hornem。)种子营养质量的两种成分的有效性:蛋白质和L-canavanine,一种有毒的非蛋白质氨基酸。 NIRS校准结果显示出良好的统计数据,总蛋白的多重测定系数(R2)为0.97,L-卡万碱为0.95。所开发的方程式进一步用于估计一组未知样品的蛋白质和L-canavanine的含量(外部验证)。总蛋白方程能够以与参考方法相似的精度进行预测,显示参考值与预测值之间的相关性(r2)为0.95。对于L-canavanine,r2仅为0.72,该方程式仅对将样品区分为低,中和高含量组有效。

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