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首页> 外文期刊>South-East European Forestry >The Potential Use of Indigobush (Amorpha fruticosa L.) as Natural Resource of Biologically Active Compounds
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The Potential Use of Indigobush (Amorpha fruticosa L.) as Natural Resource of Biologically Active Compounds

机译：靛蓝（Amorpha fruticosa L.）作为生物活性化合物的天然资源的潜在用途

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Background and Purpose: Recent research indicates that a weed like Indigobush (Amorpha fruticosa L.) gives great opportunities for its commercialization through a rich spectrum of its beneficial biological features with possible use in the forestry and biotechnology field. Therefore, in this study we wanted to explore some of potential application of Indigobush extract, as a source of biologically active compounds, for animal cell culturing as well as green corrosion inhibitors. Materials and Methods: The effect of ethanol extract of Indigobush seeds was studied on human tumor cell lines (HeLa and MCF-7) and cell viability was determined by WST-1 method after 72 hours of treatment with 6 different extract concentrations (0.5-10 mg?mL-1). The inhibition effect of Indigobush seeds extract on the corrosion of aluminum in 0.5 M hydrochloric acid solution was investigated by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) methods. Results: Results showed that the addition of Indigobush extract had a stimulatory effect on MCF-7 cells growth at the concentrations >1 mg?mL-1 while the same effect on HeLa cells was observed only at the highest concentration of Indigobush extract (10 mg?mL-1). The stimulatory effect of Indigobush extract on cell viability was more pronounced when the cells were grown in a medium with 5% FBS compared to 10% FBS (v/v). Indigobush extract did not show cytotoxic effect on MCF-7 and HeLa cells. Electrochemical studies showed that with increasing extract concentrations (2.5-15 mg?mL-1) the values of corrosion current densities decrease, while the polarization resistance values increase. The maximum inhibition efficiency of Indigobush extract is reached at concentration of 15 mg?mL-1 (82.9%). Conclusions: The Indigobush ethanol extract has no cytotoxic effect on human tumor cell lines MCF-7 and HeLa. Results confirmed that extract originated from Indigobush has the potential to utilize for the mammalian cell culture media formulation by replacing the animal serum. Furthermore, data indicates that Indigobush extract has potential as green alternative to existing synthetic corrosion inhibitors.
机译：背景与目的：最近的研究表明，像靛蓝（Indigobush）（Amorpha fruticosa L.）这样的杂草通过其有益的生物特征的丰富范围为其商业化提供了巨大的机会，并有可能在林业和生物技术领域中使用。因此，在这项研究中，我们希望探讨靛蓝提取物作为生物活性化合物的来源在动物细胞培养以及绿色腐蚀抑制剂中的潜在应用。材料和方法：研究了靛蓝种子乙醇提取物对人肿瘤细胞系（HeLa和MCF-7）的影响，并用6种不同提取物浓度（0.5-10）处理72小时后，用WST-1方法测定了细胞活力。毫克？毫升-1）。通过电势极化和电化学阻抗谱（EIS）方法研究了靛蓝种子提取物对0.5 M盐酸溶液中铝腐蚀的抑制作用。结果：结果表明，当浓度> 1 mg？mL-1时，加入靛蓝提取物对MCF-7细胞的生长具有刺激作用，而仅在最高浓度的靛蓝提取物（10 mg）下，对HeLa细胞具有相同的作用。 （mL-1）。当细胞在含5％FBS的培养基中生长时，与10％FBS（v / v）相比，靛蓝提取物对细胞活力的刺激作用更加明显。靛蓝提取物对MCF-7和HeLa细胞没有细胞毒性作用。电化学研究表明，随着萃取液浓度（2.5-15 mg？mL-1）的增加，腐蚀电流密度值降低，而极化电阻值升高。 15 mg？mL-1（82.9％）的浓度可达到靛蓝提取物的最大抑制效率。结论：靛蓝乙醇提取物对人肿瘤细胞MCF-7和HeLa无细胞毒性作用。结果证实源自靛蓝的提取物具有通过替代动物血清而用于哺乳动物细胞培养基配方的潜力。此外，数据表明靛蓝提取物有潜力作为现有合成腐蚀抑制剂的绿色替代品。

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