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首页> 外文期刊>Standards in Genomic Sciences >Metagenomes of tropical soil-derived anaerobic switchgrass-adapted consortia with and without iron
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Metagenomes of tropical soil-derived anaerobic switchgrass-adapted consortia with and without iron

机译：有铁和无铁的热带土壤衍生的厌氧柳枝switch适应财团的基因组

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Tropical forest soils decompose litter rapidly with frequent episodes of anoxia, making it likely that bacteria using alternate terminal electron acceptors (TEAs) such as iron play a large role in supporting decomposition under these conditions. The prevalence of many types of metabolism in litter deconstruction makes these soils useful templates for improving biofuel production. To investigate how iron availability affects decomposition, we cultivated feedstock-adapted consortia (FACs) derived from iron-rich tropical forest soils accustomed to experiencing frequent episodes of anaerobic conditions and frequently fluctuating redox. One consortium was propagated under fermenting conditions, with switchgrass as the sole carbon source in minimal media (SG only FACs), and the other consortium was treated the same way but received poorly crystalline iron as an additional terminal electron acceptor (SG + Fe FACs). We sequenced the metagenomes of both consortia to a depth of about 150 Mb each, resulting in a coverage of 26× for the more diverse SG + Fe FACs, and 81× for the relatively less diverse SG only FACs. Both consortia were able to quickly grow on switchgrass, and the iron-amended consortium exhibited significantly higher microbial diversity than the unamended consortium. We found evidence of higher stress in the unamended FACs and increased sugar transport and utilization in the iron-amended FACs. This work provides metagenomic evidence that supplementation of alternative TEAs may improve feedstock deconstruction in biofuel production.
机译：热带森林土壤会迅速分解凋落物，并发生频繁的缺氧事件，这使得使用铁等交替末端电子受体（TEA）的细菌在这些条件下对分解的支持起很大作用。垃圾分解中许多新陈代谢的盛行使这些土壤成为改善生物燃料生产的有用模板。为了研究铁的可用性如何影响分解，我们建立了一种适应于原料的财团（FAC），该财团是由习惯于频繁发生厌氧条件和频繁波动的氧化还原的富含铁的热带森林土壤衍生而来的。一个财团在发酵条件下繁殖，其中柳枝as是唯一培养基中的唯一碳源（仅SG FAC），另一财团的处理方式相同，但接收到的结晶铁较弱，作为额外的末端电子受体（SG + Fe FAC） 。我们对两个财团的元基因组进行了测序，每个基因组的深度约为150 Mb，对于较多样化的SG + Fe FAC，其覆盖率为26倍;对于相对较多样化的仅SG的FAC，覆盖率为81倍。两个财团都能够在柳枝quickly上快速生长，并且铁改良财团显示出比未改良财团高得多的微生物多样性。我们发现，未经修饰的FAC中存在较高的压力，而经铁修饰的FAC中糖的转运和利用增加。这项工作提供了宏基因组学证据，即补充替代的TEA可能会改善生物燃料生产中的原料解构。

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