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首页> 外文期刊>Standards in Genomic Sciences >Complete genome sequence and characterization of avian pathogenic Escherichia coli field isolate ACN001
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Complete genome sequence and characterization of avian pathogenic Escherichia coli field isolate ACN001

机译：禽病原性大肠杆菌田间分离株ACN001的完整基因组序列和表征

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Avian pathogenic Escherichia coli is an important etiological agent of avian colibacillosis, which manifests as respiratory, hematogenous, meningitic, and enteric infections in poultry. It is also a potential zoonotic threat to human health. The diverse genomes of APEC strains largely hinder disease prevention and control measures. In the current study, pyrosequencing was used to analyze and characterize APEC strain ACN001 (= CCTCC 2015182T?=?DSMZ 29979T), which was isolated from the liver of a diseased chicken in China in 2010. Strain ACN001 belongs to extraintestinal pathogenic E. coli phylogenetic group B1, and was highly virulent in chicken and mouse models. Whole genome analysis showed that it consists of six different plasmids along with a circular chromosome of 4,936,576?bp, comprising 4,794 protein-coding genes, 108 RNA genes, and 51 pseudogenes, with an average G?+?C content of 50.56?%. As well as 237 coding sequences, we identified 39 insertion sequences, 12 predicated genomic islands, 8 prophage-related sequences, and 2 clustered regularly interspaced short palindromic repeats regions on the chromosome, suggesting the possible occurrence of horizontal gene transfer in this strain. In addition, most of the virulence and antibiotic resistance genes were located on the plasmids, which would assist in the distribution of pathogenicity and multidrug resistance elements among E. coli populations. Together, the information provided here on APEC isolate ACN001 will assist in future study of APEC strains, and aid in the development of control measures.
机译：禽致病性大肠杆菌是禽大肠杆菌病的重要病原体，表现为家禽的呼吸道，血源性，脑膜炎和肠道感染。这也是对人类健康的潜在人畜共患威胁。 APEC菌株的不同基因组在很大程度上阻碍了疾病的预防和控制。在本研究中，焦磷酸测序用于分析和鉴定APEC菌株ACN001（= CCTCC 2015182T？=？DSMZ 29979T），该菌株于2010年从中国一只患病鸡的肝脏中分离出来。菌株ACN001属于肠外致病性大肠杆菌。系统发育组B1，在鸡和小鼠模型中具有高毒力。全基因组分析表明，它由六个不同的质粒以及一个4,936,576？bp的圆染色体组成，包含4,794个蛋白质编码基因，108个RNA基因和51个假基因，平均Gα+ΔC含量为50.56％。除了237个编码序列外，我们还鉴定了39个插入序列，12个预测的基因组岛，8个与噬菌体相关的序列以及2个在染色体上成簇的规则间隔的短回文重复序列区域，表明该菌株可能发生水平基因转移。另外，大多数毒力和抗生素抗性基因位于质粒上，这将有助于在大肠杆菌群体中分布致病性和多药抗性元素。总之，此处提供的有关APEC分离物ACN001的信息将有助于APEC菌株的进一步研究，并有助于制定控制措施。

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