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首页> 外文期刊>Standards in Genomic Sciences >Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site
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Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site

机译：嗜热芽孢杆菌的分离和完整的基因组序列。来自北极深海热液喷口的12AMOR1

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摘要

Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G?+?C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.
机译：地芽孢杆菌属的成员已经从世界范围内的多种生境中分离出来，并且是在各种工业应用中有针对性地利用酶的主题。在这里，我们报告了嗜热淀粉降解地芽孢杆菌的分离和完整的基因组序列。 12AMOR1。菌株12AMOR1是从Jan Mayen热液通风口站点的深海热沉积物中分离出来的。芽孢杆菌12AMOR1由一个3,410,035 bp的环状染色体和一个32,689 bp的质粒组成，它们的Gβ+ΔC含量分别为52％和47％。该基因组包含3323个蛋白质编码基因，88个tRNA种类和10个rRNA操纵子。分离物生长在一组糖，复杂多糖和蛋白质碳源上。因此，在基因组中鉴定了编码碳水化合物活性酶（CAZy）和肽酶的基因的多功能性。糖苷水解酶家族13的酶的表达，纯化和表征显示淀粉降解能力和高热稳定性，熔融温度为76.4°C。总体而言，获得的数据指出了具有巨大生物勘探潜力的海洋热液喷口的新隔离物。

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