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Draft genome sequence of Streptomyces sp. TP-A0867, an alchivemycin producer

机译:草拟链霉菌的基因组序列草案。 TP-A0867,阿奇霉素生产商

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Streptomyces sp. TP-A0867 (=NBRC 109436) produces structurally complex polyketides designated alchivemycins A and B. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation, and analysis of the genome sequence. The 9.9?Mb genome of Streptomyces sp. TP-A0867 encodes 8,385 putative ORFs, of which 7,232 were assigned with COG categories. We successfully identified a hybrid polyketide synthase (PKS)/ nonribosomal peptide synthetase (NRPS) gene cluster that could be responsible for alchivemycin biosynthesis, and propose the biosynthetic pathway. The alchivemycin biosynthetic gene cluster is also present in Streptomyces rapamycinicus NRRL 5491supT/sup, Streptomyces hygroscopicus subsp. hygroscopicus NBRC 16556, and Streptomyces ascomycinicus NBRC 13981supT/sup, which are taxonomically highly close to strain TP-A0867. This study shows a representative example that distribution of secondary metabolite genes is correlated with evolution within the genus Streptomyces .
机译:链霉菌TP-A0867(= NBRC 109436)生产结构复杂的聚酮类化合物,命名为Alchivemycins A和B。在这里,我们报告了该菌株的基因组序列草案以及该生物的特征以及装配,注释和基因组序列分析。链霉菌的9.9?Mb基因组。 TP-A0867编码8,385个假定的ORF,其中7,232个已分配COG类别。我们成功地确定了杂合聚酮化合物合酶(PKS)/非核糖体肽合成酶(NRPS)基因簇,可以负责白霉素的生物合成,并提出了生物合成途径。雷奇链霉菌NRRL 5491 T ,吸水链霉菌亚种中也存在白霉素生物合成基因簇。吸湿性NBRC 16556和子链霉菌NBRC 13981 T ,它们在分类学上非常接近菌株TP-A0867。这项研究显示了一个代表性的例子,次级代谢产物基因的分布与链霉菌属内的进化有关。

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