...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Stem Cell Reports >A Simple Bioreactor-Based Method to Generate Kidney Organoids from?Pluripotent Stem Cells
【24h】

A Simple Bioreactor-Based Method to Generate Kidney Organoids from?Pluripotent Stem Cells

机译：一种基于生物反应器的简单方法，可从多能干细胞生成肾脏类器官。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Summary Kidney organoids made from pluripotent stem cells have the potential to revolutionize how kidney development, disease, and injury are studied. Current protocols are technically complex, suffer from poor reproducibility, and have high reagent costs that restrict scalability. To overcome some of these issues, we have established a simple, inexpensive, and robust method to grow kidney organoids in bulk from human induced pluripotent stem cells. Our organoids develop tubular structures by day 8 and show optimal tissue morphology at day 14. A comparison with fetal human kidneys suggests that day-14 organoid tissue most closely resembles late capillary loop stage nephrons. We show that deletion of HNF1B , a transcription factor linked to congenital kidney defects, interferes with tubulogenesis, validating our experimental system for studying renal developmental biology. Taken together, our protocol provides a fast, efficient, and cost-effective method for generating large quantities of human fetal kidney tissue, enabling the study of normal and aberrant kidney development.
机译：总结由多能干细胞制成的肾脏类器官有潜力改变研究肾脏发育，疾病和损伤的方式。当前的方案在技术上很复杂，可重复性差，并且试剂成本高，限制了可扩展性。为了克服这些问题中的一些，我们已经建立了一种简单，廉价且健壮的方法来从人诱导的多能干细胞中大量生长肾脏类器官。我们的类器官在第8天发育成管状结构，并在第14天显示出最佳的组织形态。与胎儿肾脏的比较表明，第14天的类器官组织最接近晚期毛细血管loop期肾。我们显示，HNF1B（与先天性肾脏缺陷相关的转录因子）的缺失会干扰肾小管生成，从而验证了我们研究肾脏发育生物学的实验系统。综上所述，我们的方案为生成大量人胎儿肾脏组织提供了一种快速，高效且经济高效的方法，从而能够研究正常和异常的肾脏发育。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号